Evidence map›Paper›PMID 31324218›Full record

ArticleJournal for immunotherapy of cancer2019

Ovarian cancer stem cells and macrophages reciprocally interact through the WNT pathway to promote pro-tumoral and malignant phenotypes in 3D engineered microenvironments.

Shreya Raghavan, Pooja Mehta, Yuying Xie, Yu L Lei, Geeta Mehta

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal for immunotherapy of cancer, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 152 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
152citing papers in PubMed, 1 pooled it
8.4field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

152 citing papers in PubMed, 1 synthesis or guideline pooled it, 233 citations in OpenAlex.

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92 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 2 countries.

Shreya RaghavanDepartment of Materials Science and Engineering, 2800 Plymouth Rd, Building 28, Room 3044W, Ann Arbor, MI, 48109, USA.
Pooja MehtaDepartment of Materials Science and Engineering, 2800 Plymouth Rd, Building 28, Room 3044W, Ann Arbor, MI, 48109, USA.
Yuying XieDepartment of Computational Mathematics, Science, and Engineering, Michigan State University, East Lansing, MI, 48823, USA.
Yu L LeiDepartment of Periodontics and Oral Medicine and Department of Otolaryngology Head and Neck Surgery, Ann Arbor, USA.
Geeta MehtaDepartment of Materials Science and Engineering, 2800 Plymouth Rd, Building 28, Room 3044W, Ann Arbor, MI, 48109, USA. mehtagee@umich.edu.ORCID 0000-0001-5967-6425
University of Michigan · USMichigan State University · US

Funding

XenograftP30CA046592 · NCI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI THEODORE S LAWRENCE · 1988 to 2026
$178.2M
Tissue Engineering and RegenerationT32DE007057 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI DAVID H. KOHN · 1985 to 2026
$17.3M
Restoring the Immunogenicity of Head and Neck CancerR01DE026728 · NIDCR · UNIVERSITY OF TX MD ANDERSON CAN CTR · PI Yu Leo Lei, James J. Moon · 2018 to 2026
$4.3M
Autophagy-promoting NLRX1-TUFM complex and cancer cell resistance to cetuximabR00DE024173 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI LEI, YU LEO · 2016 to 2018
$863k
NCI NIH HHS P30 CA046592NIDCR NIH HHS R00 DE024173NIDCR NIH HHS R01 DE026728NIDCR NIH HHS T32 DE007057
6 · The paper itself

Abstract

backgroundInnate immune cells such as macrophages are abundantly present within malignant ascites, where they share the microenvironment with ovarian cancer stem cells (CSC).

methodsTo mimic this malignant ascites microenvironment, we created a hanging-drop hetero-spheroid model to bring CSCs and macrophages in close association. Within these hetero-spheroids, CD68

resultsOur results indicate that CSCs drive the upregulation of M2 macrophage marker CD206 within hetero-spheroids, compared to bulk ovarian cancer cells, implying an inherently more immuno-suppressive program. Moreover, an increased maintenance of elevated aldehyde dehydrogenase (ALDH) activity is noted within hetero-spheroids that include pre-polarized CD206

conclusionsOur data implies that macrophage- initiated WNT signaling could play a significant role in the maintenance of stemness, and the resulting phenotypes of chemoresistance and invasiveness. Our results indicate paracrine WNT activation during CSC/M2 macrophages interaction constitutes a positive feedback loop that likely contributes to the more aggressive phenotype, which makes the WNT pathway a potential target to reduce the CSC and M2 macrophage compartments in the tumor microenvironment.

Indexed as

Wnt Signaling PathwayAnimalsCell Line, TumorCoculture TechniquesFemaleGene Expression Regulation, NeoplasticHumansMacrophagesMiceNeoplasm TransplantationNeoplastic Stem CellsOvarian NeoplasmsPhenotypeSpheroids, CellularTumor Microenvironment

Identifiers

PMID31324218
PMCPMC6642605
OpenAlexW2964278761

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.