ArticlePloS one2019
Microstructure and in-depth proteomic analysis of Perna viridis shell.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 30 citations in OpenAlex.
- Diversification of von Willebrand Factor A and Chitin-Binding Domains in Pif/BMSPs Among Mollusks.Journal of molecular evolution · 2024Article
- The first high-density genetic map of common cockle (Cerastoderma edule) reveals a major QTL controlling shell color variation.Scientific reports · 2022Article
- The Mantle Transcriptome ofAnimals : an open access journal from MDPI · 2022Article
- Optimized Sensory Units Integrated in the Chiton Shell.Marine biotechnology (New York, N.Y.) · 2022Article
- Contamination gradient affects differently carbonic anhydrase activity of mollusks depending on their feeding habits.Ecotoxicology (London, England) · 2022Article
- Hydrophilic Shell Matrix Proteins ofGenes · 2021Article
- PU14, a Novel Matrix Protein, Participates in Pearl Oyster, Pinctada Fucata, Shell Formation.Marine biotechnology (New York, N.Y.) · 2021Article
- Diversified Biomineralization Roles ofInternational journal of molecular sciences · 2021Article
- The 'Shellome' of the Crocus ClamFrontiers in genetics · 2021Article
- Recombinant transgelin-like protein 1 from Mytilus shell induces formation of CaCOFEBS open bio · 2020Article
- Ps19, a novel chitin binding protein from Pteria sterna capable to mineralize aragonite plates in vitro.PloS one · 2020Article
- Pearl Sac Gene Expression Profiles Associated With Pearl Attributes in the Silver-Lip Pearl Oyster,Frontiers in genetics · 2020Article
- Molecular Characterization of a Novel Shell Matrix Protein With PDZ Domain FromFrontiers in physiology · 2020Article
- Molecular characterization of a whirlin-like protein with biomineralization-related functions from the shell of Mytilus coruscus.PloS one · 2020Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For understanding the structural characteristics and the proteome of Perna shell, the microstructure, polymorph, and protein composition of the adult Perna viridis shell were investigated. The P. viridis shell have two distinct mineral layers, myostracum and nacre, with the same calcium carbonate polymorph of aragonite, determined by scanning electron microscope, Fourier transform infrared spectroscopy, and x-ray crystalline diffraction. Using Illumina sequencing, the mantle transcriptome of P. viridis was investigated and a total of 69,859 unigenes was generated. Using a combined proteomic/transcriptomic approach, a total of 378 shell proteins from P. viridis shell were identified, in which, 132 shell proteins identified with more than two matched unique peptides. Of the 132 shell proteins, 69 are exclusive to the nacre, 12 to the myostracum, and 51 are shared by both. The Myosin-tail domain containing proteins, Filament-like proteins, and Chitin-binding domain containing proteins represent the most abundant molecules. In addition, the shell matrix proteins (SMPs) containing biomineralization-related domains, such as Kunitz, A2M, WAP, EF-hand, PDZ, VWA, Collagen domain, and low complexity regions with abundant certain amino acids, were also identified from P. viridis shell. Collagenase and chitinase degradation can significantly change the morphology of the shell, indicating the important roles of collagen and chitin in the shell formation and the muscle-shell attachment. Our results present for the first time the proteome of P. viridis shell and increase the knowledge of SMPs in this genus.
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