Evidence map›Paper›PMID 31321735›Full record

ArticleGenes & genomics2019

(Cyto)genomic and epigenetic characterization of BICR 10 cell line and three new established primary human head and neck squamous cell carcinoma cultures.

Ilda P Ribeiro, Joana M Rodrigues, Alexandra Mascarenhas, Vanessa Marques, Francisco Caramelo, Maria J Julião, Thomas Liehr, Joana B Melo, Isabel M Carreira

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Article in Genes & genomics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Ilda P RibeiroCytogenetics and Genomics Laboratory, Faculty of Medicine, University of Coimbra, Polo Ciências da Saúde, 3000-354, Coimbra, Portugal.
Joana M RodriguesCytogenetics and Genomics Laboratory, Faculty of Medicine, University of Coimbra, Polo Ciências da Saúde, 3000-354, Coimbra, Portugal.
Alexandra MascarenhasCytogenetics and Genomics Laboratory, Faculty of Medicine, University of Coimbra, Polo Ciências da Saúde, 3000-354, Coimbra, Portugal.
Vanessa MarquesCytogenetics and Genomics Laboratory, Faculty of Medicine, University of Coimbra, Polo Ciências da Saúde, 3000-354, Coimbra, Portugal.
Francisco CarameloLaboratory of Biostatistics and Medical Informatics, IBILI-Faculty of Medicine, University of Coimbra, 3000-354, Coimbra, Portugal.
Maria J JuliãoDepartment of Patholog, Coimbra Hospital and University Centre, CHUC, EPE, 3000-075, Coimbra, Portugal.
Thomas LiehrInstitute of Human Genetics, Jena University Hospital, Friedrich Schiller University, Jena, Germany.
Joana B MeloCytogenetics and Genomics Laboratory, Faculty of Medicine, University of Coimbra, Polo Ciências da Saúde, 3000-354, Coimbra, Portugal.
Isabel M CarreiraCytogenetics and Genomics Laboratory, Faculty of Medicine, University of Coimbra, Polo Ciências da Saúde, 3000-354, Coimbra, Portugal. citogenetica@fmed.uc.pt.

Funding

ATTENTION, COGNITION AND DRIVING PERFORMANCE IN SDATR01AG010145 · NIA · JEWISH HOSPITAL OF SAINT LOUIS · PI MORRIS, JOHN · 1992 to 1994
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6 · The paper itself

Abstract

backgroundHead and neck squamous cell carcinoma cell lines are useful preclinical models to understand the molecular processes underlying the development of such tumors, and to establish targeted therapies.

objectiveWe performed a comprehensive (cyto)genomic and epigenetic characterization of three new established primary human head and neck squamous cell carcinoma cultures and an established, yet undercharacterized cell line: BICR 10.

methodsKaryotyping, multiplex fluorescence in situ hybridization, array comparative genomic hybridization and methylation-specific multiplex ligation-dependent probe amplification were applied.

resultsThe three primary cultures turned out to be a near-triploid and BICR 10 near-diploid. Banding and molecular cytogenetic analysis revealed non-random numerical and structural aberrations. The most common rearrangements identified in BICR 10 cell line were non-complex derivatives of reciprocal translocations, in which the breakpoints often appeared in centromeric/near-centromeric regions. In the 3 primary cell cultures the most common rearrangements observed were iso- and derivatives chromosomes derived from translocations. Overall, gains of 7p, 8q and losses at 3p, 8p, 9p, 18q and Xp were present in all four studied samples. Among the analyzed genes, BICR 10 cell line exhibited enhanced methylation of gene promoter; however, in all studied samples PAX5, WT1 and GATA5 were methylated.

conclusionThe here reported comprehensive characterization of BICR 10 cell line and the new established cultures enriches the resources available for head and neck cancer research, especially for testing therapeutic agents.

Indexed as

Cell Line, TumorEpigenesis, GeneticGenomicsCell Culture TechniquesChromosome AberrationsChromosome BandingComparative Genomic HybridizationDNA MethylationHead and Neck NeoplasmsHumansIn Situ Hybridization, FluorescenceKaryotypingSquamous Cell Carcinoma of Head and NeckBICR 10 cell lineChromosomal rearrangementsHead and neck cancerMethylationPrimary cell cultures

Identifiers

PMID31321735

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.