Evidence map›Paper›PMID 31321732›Full record

ArticleJournal of cell communication and signaling2019

Chronic shisha exposure alters phosphoproteome of oral keratinocytes.

Shankargouda Patil, Pavithra Rajagopalan, Krishna Patel, Tejaswini Subbannayya, Niraj Babu, Sonali V Mohan, Jayshree Advani, Gajanan Sathe, Shilpa Bhandi, Hitendra S Solanki and 4 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cell communication and signaling, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed, 1 pooled it
0.5field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 1 synthesis or guideline pooled it, 6 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 5 institutions in 6 countries.

Shankargouda PatilDepartment of Maxillofacial Surgery and Diagnostic Sciences, Division of Oral Pathology, College of Dentistry, Jazan University, Jazan, Saudi Arabia.
Pavithra RajagopalanInstitute of Bioinformatics, International Technology Park, Bangalore, 560066, India.
Krishna PatelInstitute of Bioinformatics, International Technology Park, Bangalore, 560066, India.
Tejaswini SubbannayyaInstitute of Bioinformatics, International Technology Park, Bangalore, 560066, India.
Niraj BabuInstitute of Bioinformatics, International Technology Park, Bangalore, 560066, India.
Sonali V MohanInstitute of Bioinformatics, International Technology Park, Bangalore, 560066, India.
Jayshree AdvaniInstitute of Bioinformatics, International Technology Park, Bangalore, 560066, India.
Gajanan SatheInstitute of Bioinformatics, International Technology Park, Bangalore, 560066, India.
Shilpa BhandiDepartment of Restorative Dental Sciences, Division of Operative Dentistry, College of Dentistry, Jazan University, Jazan, Saudi Arabia.
Hitendra S SolankiInstitute of Bioinformatics, International Technology Park, Bangalore, 560066, India.
David SidranskyDepartment of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Aditi ChatterjeeInstitute of Bioinformatics, International Technology Park, Bangalore, 560066, India.
Harsha GowdaInstitute of Bioinformatics, International Technology Park, Bangalore, 560066, India. harsha@ibioinformatics.org.ORCID http://orcid.org/0000-0002-4118-6855
Marco FerrariDepartment of Medical Biotechnologies, School of Dental Medicine, University of Siena, Siena, Italy. ferrarm@gmail.com.
Institute of Bioinformatics · INManipal Academy of Higher Education · INUniversity of Siena · ITJazan University · SAJohns Hopkins University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Shisha smoking has been epidemiologically linked to oral cancer. However, few studies have investigated the pathobiology of shisha-induced cellular transformation. We studied the effects of chronic shisha exposure (8 months) in an in vitro model using immortalized, non-neoplastic oral keratinocytes (OKF6/TERT1). Quantitative proteomic and phosphoproteomic analyses were performed on OKF6/TERT1 cells treated with shisha extract for a period of 8 months. Pathway analysis was carried out to identify significantly enriched biological processes in shisha-treated cells. Chronic shisha exposure resulted in increased cell scattering phenomenon in OKF6/TERT1 cells. Data analysis revealed differential phosphorylation of 164 peptides (fold change ≥1.5, p ≤ 0.0.5) corresponding to 136 proteins. Proteins associated with mTORC1 and EIF4F complexes involved in initiating protein translation were seen to be enriched upon shisha treatment. Network analysis also highlighted downregulation of proteins involved in Type I interferon signaling in shisha-treated cells. Quantitative phosphoproteomic approach elucidated global perturbations to the molecular milieu of oral keratinocytes upon shisha exposure. Further studies are needed to validate putative targets in oral cancer patients with shisha smoking history.

Indexed as

High-throughputHookahNarghileOrbitrap fusionWaterpipe

Identifiers

PMID31321732
PMCPMC6732141
OpenAlexW2956406792

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.