ArticleNature chemical biology2019
A programmable DNA-origami platform for studying lipid transfer between bilayers.
Article in Nature chemical biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers, 1 of them a synthesis that pooled it.
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Who cites it
45 citing papers in PubMed, 1 synthesis or guideline pooled it, 100 citations in OpenAlex.
- Stay in touch with the endoplasmic reticulum.Science China. Life sciences · 2024Pooled it
- Advanced imaging of membrane contact sites.Nature cell biology · 2026Review
- A synthetic cell microreactor with two types of interacting dynamic DNA-based pores.Nature chemistry · 2026Article
- Lipid traffic: an enigmatic alliance between ORPs and a TMEM16-like protein at membrane contact sites.Biochemical Society transactions · 2026Review
- Vesicle-Templated Self-Assembly of Programmable Freestanding Multi-μm DNA Shells.Nano letters · 2026Article
- Ist2 promotes lipid transfer by Osh6 via its membrane tethering and lipid scramblase activities.Science advances · 2025Article
- Programmable Liposome Organization via DNA Origami Templates.Journal of the American Chemical Society · 2025Article
- Single-Molecule Detection of Optical Signals Using DNA-Based Plasmonic Nanostructures.Biosensors · 2025Review
- Uncovering the Mechanisms of Intracellular Membrane Trafficking by Reconstituted Membrane Systems.Membranes · 2025Review
- Multiple cAMP/PKA complexes at the STIM1 ER/PM junction specified by E-Syt1 and E-Syt2 reciprocally gates ANO1 (TMEM16A) via CaNature communications · 2025Article
- A Practical Approach for Polarity and Quantity Controlled Assembly of Membrane Proteins into Nanoliposomes.Chembiochem : a European journal of chemical biology · 2025Article
- Functional Nucleic Acid Nanostructures for Mitochondrial Targeting: The Basis of Customized Treatment Strategies.Molecules (Basel, Switzerland) · 2025Review
- Role of lipids in interorganelle communication.Trends in cell biology · 2025Review
- DNA-Assisted Assays for Studying Lipid Transfer Between Membranes.Methods in molecular biology (Clifton, N.J.) · 2025Article
- A DNA Origami Bubble Blower for Liposome Production.ACS omega · 2024Article
- Lipid osmosis, membrane tension, and other mechanochemical driving forces of lipid flow.Current opinion in cell biology · 2024Review
- Lipid osmosis, membrane tension, and other mechanochemical driving forces of lipid flow.bioRxiv : the preprint server for biology · 2024Article
- Triggered contraction of self-assembled micron-scale DNA nanotube rings.Nature communications · 2024Article
- High spatial-resolved heat manipulating membrane heterogeneity alters cellular migration and signaling.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Non-vesicular lipid transport between bilayers at membrane contact sites plays important physiological roles. Mechanistic insight into the action of lipid-transport proteins localized at these sites requires determination of the distance between bilayers at which this transport can occur. Here we developed DNA-origami nanostructures to organize size-defined liposomes at precise distances and used them to study lipid transfer by the synaptotagmin-like mitochondrial lipid-binding protein (SMP) domain of extended synaptotagmin 1 (E-Syt1). Pairs of DNA-ring-templated donor and acceptor liposomes were docked through DNA pillars, which determined their distance. The SMP domain was anchored to donor liposomes via an unstructured linker, and lipid transfer was assessed via a Förster resonance energy transfer (FRET)-based assay. We show that lipid transfer can occur over distances that exceed the length of an SMP dimer, which is compatible with the shuttle model of lipid transport. The DNA nanostructures developed here can also be adapted to study other processes occurring where two membranes are closely apposed to each other.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.