ArticleScientific reports2019
Co-cultivation of the marine sponge Halichondria panicea and its associated microorganisms.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 30 citations in OpenAlex.
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- A diffusion-based integrative approach for culturing previously uncultured bacteria from marine sediments.Marine life science & technology · 2025Article
- Immunomodulatory Compounds from the Sea: From the Origins to a Modern Marine Pharmacopoeia.Marine drugs · 2024Review
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- Paracoccus onchidii sp. nov., a moderately halophilic bacterium isolated from a marine invertebrate from the South China Sea.Antonie van Leeuwenhoek · 2023Article
- Synthase-selected sorting approach identifies a beta-lactone synthase in a nudibranch symbiotic bacterium.Microbiome · 2023Article
- Conservation of Genomic Information in Multiple Displacement Amplified Low-Quantity Metagenomic Material from Marine Invertebrates.Marine drugs · 2023Article
- Reaching unreachables: Obstacles and successes of microbial cultivation and their reasons.Frontiers in microbiology · 2023Review
- Review
- Light Availability Affects the Symbiosis of Sponge Specific Cyanobacteria and the Common Blue Aquarium Sponge (Animals : an open access journal from MDPI · 2022Article
- Factors affecting the isolation and diversity of marine sponge-associated bacteria.Applied microbiology and biotechnology · 2022Article
- Cultivation and diversity analysis of novel marine thraustochytrids.Marine life science & technology · 2021Review
- Triggering Growth via Growth Initiation Factors in Nature: A Putative Mechanism forFrontiers in microbiology · 2021Article
- Bacteria Cultivated From Sponges and Bacteria Not Yet Cultivated From Sponges-A Review.Frontiers in microbiology · 2021Review
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Authors and funding
3 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Marine sponges host bacterial symbionts with biotechnological potential, yet isolation of true sponge symbionts remains difficult due to their host dependency. Moreover, attempts to grow sponges for their pharmacologically-active compounds outside of their habitat often results in a shift of their microbial community. In this study we evaluate suitable sponge cultivation methods that allow maintenance of both the marine sponge Halichondria panicea and its associated bacteria in an ex situ environment. In addition, we present a method for co-cultivation of sponge explants and microbes separated by a membrane in a multi-chamber device. Tests on ex situ cultivation of H. panicea under different controlled conditions showed that only high water exchange rates in the aquarium enabled maintenance of its dominant symbiont "Candidatus Halichondribacter symbioticus" at a high relative abundance in the sponge body, a prerequisite for co-cultivation. The bacterial enrichment retrieved from co-cultivation contained bacteria from nine different classes in addition to sequences corresponding to "Ca. H. symbioticus". This represents an increase of the cultivable bacterial classes from H. panicea compared to standard isolation techniques on solid media plates. The current study provides insights into sponge-microbe maintenance under ex situ conditions and proposes a new method for the isolation of sponge-associated bacteria.
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