ArticleBMC pulmonary medicine2019
Multiplex PCR of bronchoalveolar lavage fluid in children enhances the rate of pathogen detection.
Article in BMC pulmonary medicine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 2 of them syntheses that pooled it.
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Who cites it
14 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Accuracy of Molecular Amplification Assays for Diagnosis of Staphylococcal Pneumonia: a Systematic Review and Meta-analysis.Journal of clinical microbiology · 2021Pooled it
- Pooled it
- Bronchoalveolar lavage in pediatric patients: are we doing it right?ATS scholar · 2026Review
- Risk factors for plastic bronchitis in children with community-acquired pneumonia: a retrospective bronchoscopy-based study.BMC pulmonary medicine · 2026Article
- The evolution of next-generation lateral flow assays for bacterial and fungal diagnostics.Mikrochimica acta · 2026Review
- A Novel PCR Panel for Bacterial Detection in Lower Respiratory Tract Infections: A Comparative Study with Culture Results.Pathogens (Basel, Switzerland) · 2025Article
- Clinical characteristics of severe community-acquired pneumonia in children with virus mono-detection versus co-detection with bacteria.BMC pulmonary medicine · 2025Article
- Determination and Comparison of the Pathogen Spectrum Evaluated by Microbial Culture and Multiplex PCR During Bronchoscopy with Regard to Clinical Utility of Routine Bronchial Wash in Patients with Various Pulmonary Diseases.Diagnostics (Basel, Switzerland) · 2025Article
- Lung infections in immunocompromised children.Pediatric radiology · 2024Review
- Diagnostic performance of metagenomic next-generation sequencing for Pneumocystis jirovecii pneumonia.BMC infectious diseases · 2023Review
- Article
- Multiplex bacterial PCR in the bronchoalveolar lavage fluid of non-intubated patients with suspected pulmonary infection: a quasi-experimental study.ERJ open research · 2022Article
- Effect of Risk-Focused Diversified Safety Management Mode in Patients with Major Artery Stent Implantation.Emergency medicine international · 2022Article
- Respiratory microbes detected in hospitalized adults with acute respiratory infections: associations between influenza A(H1N1)pdm09 virus and intensive care unit admission or fatal outcome in Vietnam (2015-2017).BMC infectious diseases · 2021Article
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8 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundCulturing of bronchoalveolar lavage (BAL) fluid is a commonly used method for pathogen detection in pneumonia. However, the sensitivity is low, especially in patients pre-treated with anti-infective agents. The early detection of a pathogen is crucial for the outcome of respiratory tract infections. For bloodstream infections, a multiplex polymerase chain reaction (PCR) assay (SeptiFast®, SF) is available for improved pathogen detection from blood.
objectiveThe aim of the present study was to determine whether the SF assay is applicable to the BAL of children with pulmonary infections and whether the frequency of pathogen detection is enhanced by the use of this multiplex PCR method.
methodsWe investigated 70 BAL samples of 70 children simultaneously by culture and multiplex PCR. The frequency of pathogen detection was compared.
resultsPathogens were detected more frequently by SF than by culture (83% vs. 31%; p < 0.001). This advantage was shown for immunocompetent patients (p = 0.001) as well as for immunocompromised patients (p = 0.003). The majority (38/44; 86%) of the Gram positive cocci were only detected by SF. Fungal organisms were detected in 7/70 patients (10%) by SF and in 2/70 (3%) by culture (p = 0.125).
conclusionCompared to conventional culture, the use of the SF assay on the BAL of children with pneumonia increases pathogen detection rates and therefore adds important information to guide anti-infective therapy.
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