ArticleBMC plant biology2019
De novo transcriptome sequencing and gene expression profiling of Magnolia wufengensis in response to cold stress.
Article in BMC plant biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 95 citations in OpenAlex.
- Integrated Transcriptome and Proteome Analysis Provides Insights into the Mechanism ofBiology · 2026Article
- Physiological Responses ofPlants (Basel, Switzerland) · 2025Article
- Gene Expression Profiling of Transcription Factors and Acclimation-Related Genes inInternational journal of molecular sciences · 2025Article
- Study on the physiological mechanism and molecular regulatory network of Blumea balsamifera in response to drought stress.BMC plant biology · 2025Article
- Contrasting roles of reactive oxygen species in pyrophosphate-induced growth inhibition under normal and salt stress conditions.Plant molecular biology · 2025Article
- Predictive Framework Based on GBIF and WorldClim Data for Identifying Drought- and Cold-TolerantPlants (Basel, Switzerland) · 2025Article
- Tissue-specific transcriptomic analysis reveals the molecular mechanisms responsive to cold stress in Poa crymophila, and development of EST-SSR markers linked to cold tolerance candidate genes.BMC plant biology · 2025Article
- Transcriptome Analysis Provides Insights into the Safe Overwintering of Local Peach Flower Buds.Current issues in molecular biology · 2024Article
- Transcriptomic and Physiological Studies Unveil that Brassinolide Maintains the Balance of Maize's Multiple Metabolisms under Low-Temperature Stress.International journal of molecular sciences · 2024Article
- Transcriptomic Analysis Reveals Adaptive Evolution and Conservation Implications for the EndangeredGenes · 2024Article
- Integrated transcriptomic and metabolomic analysis provides insights into cold tolerance in lettuce (Lactuca sativa L.).BMC plant biology · 2024Article
- Comparative analysis of POD genes and their expression under multiple hormones in Pyrus bretschenedri.BMC genomic data · 2024Article
- Transcriptomic and physiological analysis of the response of Spirodela polyrrhiza to sodium nitroprusside.BMC plant biology · 2024Article
- Comparative Transcriptomic Analysis of the Metabolism of Betalains and Flavonoids in Red Amaranth Hypocotyl under Blue Light and Dark Conditions.Molecules (Basel, Switzerland) · 2023Article
- Transcriptome Analysis Reveals Brassinolide Signaling Pathway Control of Foxtail Millet Seedling Starch and Sucrose Metabolism under Freezing Stress, with Implications for Growth and Development.International journal of molecular sciences · 2023Article
- Article
- Transcriptome analysis revealed molecular basis of cold response inMolecular breeding : new strategies in plant improvement · 2023Article
- Comparative transcriptome analysis reveals the regulatory mechanisms of two tropical water lilies in response to cold stress.BMC genomics · 2023Article
- Long-term cold, freezing and drought: overlapping and specific regulatory mechanisms and signal transduction in tea plant (Frontiers in plant science · 2023Article
- Transcriptional Memory inPlants (Basel, Switzerland) · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundMagnolia wufengensis is a new species of Magnolia L. and has considerable ornamental and economic value due to its unique characteristics. However, because of its characteristic of poor low temperature resistance, M. wufengensis is hardly popularization and application in the north of China. Furthermore, the mechanisms of gene regulation and signaling pathways involved in the cold-stress response remained unclear in this species. In order to solve the above-mentioned problems, we performed de novo transcriptome assembly and compared the gene expression under the natural (25 °C) and cold (4 °C) conditions for M. wufengensis seedlings.
resultsMore than 46 million high-quality clean reads were produced from six samples (RNA was extracted from the leaves) and were used for performing de novo transcriptome assembly. A total of 59,764 non-redundant unigenes with an average length of 899 bp (N50 = 1,110) were generated. Among these unigenes, 31,038 unigenes exhibited significant sequence similarity to known genes, as determined by BLASTx searches (E-value ≤1.0E-05) against the Nr, SwissProt, String, GO, KEGG, and Cluster of COG databases. Based on a comparative transcriptome analysis, 3,910 unigenes were significantly differentially expressed (false discovery rate [FDR] < 0.05 and |log
conclusionWe generated a genome-wide transcript profile of M. wufengensis and a de novo-assembled transcriptome that can be used to analyze genes involved in biological processes. In this study, we provide the first report of transcriptome sequencing of cold-stressed M. wufengensis. Our findings provide important clues not only for understanding the molecular mechanisms of cold stress in plants but also for introducing cold hardiness into M. wufengensis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.