Evidence map›Paper›PMID 31319790›Full record

ArticleBMC microbiology2019

Universal membrane-labeling combined with expression of Katushka far-red fluorescent protein enables non-invasive dynamic and longitudinal quantitative 3D dual-color fluorescent imaging of multiple bacterial strains in mouse intestine.

Oula Peñate-Medina, Robert J Tower, Tuula Peñate-Medina, Olga Will, Per E J Saris, Juho Suojanen, Timo Sorsa, Laura Huuskonen, Kaisa Hiippala, Reetta Satokari and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.3field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 11 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 4 institutions in 4 countries.

Oula Peñate-MedinaMolecular Imaging North Competence Center, Section Biomedical Imaging, Department of Radiology and Neuroradiology, University Hospital Schleswig-Holstein, Am Botanischen Garten 14, 24118, Kiel, Germany.
Robert J TowerMolecular Imaging North Competence Center, Section Biomedical Imaging, Department of Radiology and Neuroradiology, University Hospital Schleswig-Holstein, Am Botanischen Garten 14, 24118, Kiel, Germany.
Tuula Peñate-MedinaMolecular Imaging North Competence Center, Section Biomedical Imaging, Department of Radiology and Neuroradiology, University Hospital Schleswig-Holstein, Am Botanischen Garten 14, 24118, Kiel, Germany.
Olga WillMolecular Imaging North Competence Center, Section Biomedical Imaging, Department of Radiology and Neuroradiology, University Hospital Schleswig-Holstein, Am Botanischen Garten 14, 24118, Kiel, Germany.
Per E J SarisDepartment of Food and Environmental Sciences, University of Helsinki, Viikinkaari 9, 00014, Helsinki, Finland.
Juho SuojanenCleft Palate and Craniofacial Centre, Department of Plastic Surgery, Helsinki University Hospital, Helsinki University Central Hospital, Topeliuksenkatu 5, 00029, Helsinki, Finland.
Timo SorsaDepartment of Oral and Maxillofacial Diseases, University of Helsinki and Helsinki University Hospital, Haartmaninkatu 4E, 00029, Helsinki, Finland.
Laura HuuskonenDepartment of Bacteriology and Immunology and Immunobiology Research Program, Faculty of Medicine, University of Helsinki, Haartmaninkatu 2, 00014, Helsinki, Finland.
Kaisa HiippalaDepartment of Bacteriology and Immunology and Immunobiology Research Program, Faculty of Medicine, University of Helsinki, Haartmaninkatu 2, 00014, Helsinki, Finland.
Reetta SatokariDepartment of Bacteriology and Immunology and Immunobiology Research Program, Faculty of Medicine, University of Helsinki, Haartmaninkatu 2, 00014, Helsinki, Finland.
Claus C GlüerMolecular Imaging North Competence Center, Section Biomedical Imaging, Department of Radiology and Neuroradiology, University Hospital Schleswig-Holstein, Am Botanischen Garten 14, 24118, Kiel, Germany.
Willem M de VosDepartment of Bacteriology and Immunology and Immunobiology Research Program, Faculty of Medicine, University of Helsinki, Haartmaninkatu 2, 00014, Helsinki, Finland.
Justus ReunanenBiocenter Oulu & Cancer and Translational Medicine Research Unit, University of Oulu, Aapistie 5, 90220, Oulu, Finland. justus.reunanen@oulu.fi.ORCID 0000-0002-4217-5672
University of Helsinki · FIUniversity Hospital Schleswig-Holstein · DEHelsinki University Hospital · FIUniversity of Oulu · FI

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe human gastrointestinal (GI) tract microbiota has been a subject of intense research throughout the 3rd Millennium. Now that a general picture about microbiota composition in health and disease is emerging, questions about factors determining development of microbiotas with specific community structures will be addressed. To this end, usage of murine models for colonization studies remains crucial. Optical in vivo imaging of either bioluminescent or fluorescent bacteria is the basis for non-invasive detection of intestinal colonization of bacteria. Although recent advances in in vivo fluorescence imaging have overcome many limitations encountered in bioluminescent imaging of intestinal bacteria, such as requirement for live cells, high signal attenuation and 2D imaging, the method is still restricted to bacteria for which molecular cloning tools are available.

resultsHere, we present usage of a lipophilic fluorescent dye together with Katushka far-red fluorescent protein to establish a dual-color in vivo imaging system to monitor GI transit of different bacterial strains, suitable also for strains resistant to genetic labeling. Using this system, we were able to distinguish two different E. coli strains simultaneously and show their unique transit patterns. Combined with fluorescence molecular tomography, these distinct strains could be spatially and temporally resolved and quantified in 3D.

conclusionsDeveloped novel method for labeling microbes and identify their passage both temporally and spatially in vivo makes now possible to monitor all culturable bacterial strains, also those that are resistant to conventional genetic labeling.

Indexed as

AnimalsEscherichia coliFluorescent DyesGastrointestinal MicrobiomeGastrointestinal TractIntravital MicroscopyLuminescent ProteinsMembrane LipidsMiceMicroscopy, FluorescenceRed Fluorescent ProteinStaining and LabelingTomography, OpticalFluorescent DyesLuminescent ProteinsMembrane LipidsRed Fluorescent ProteinBacterial colonizationDual-color imagingFar-red fluorescent proteinFluorescence imagingFluorescence molecular tomographyIntestineIn vivoKatushkaMurine modelThree-dimensional

Identifiers

PMID31319790
PMCPMC6639909
OpenAlexW2957365081

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.