Evidence map›Paper›PMID 31319038›Full record

ArticleJDR clinical and translational research2019

Associations of

T H Harris, M R Wallace, H Huang, H Li, L M Shaddox

Registry-linked trialOpen access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in JDR clinical and translational research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT01330719 (Mechanisms and Treatment Response of Aggressive Periodontitis in Children), which is not on this map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact, top 94% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT01330719 nacompletednot on this map

Mechanisms and Treatment Response of Aggressive Periodontitis in Children: Aberrant Immunological Phenotypes/Functions in the Progression of AgP

TypeinterventionalSponsorUniversity of FloridaRan2006 to 2022Enrolled514ConditionsAggressive PeriodontitisArmsDiseased periodontal treatment, Conventional periodontal treatment
3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

T H HarrisDepartment of Periodontology, College of Dentistry, University of Florida, Gainesville, FL, USA.
M R WallaceDepartment of Molecular Genetics and Microbiology, College of Medicine, University of Florida, Gainesville, FL, USA.
H HuangDepartment of Periodontology, College of Dentistry, University of Florida, Gainesville, FL, USA.
H LiDepartment of Molecular Genetics and Microbiology, College of Medicine, University of Florida, Gainesville, FL, USA.
L M ShaddoxDepartment of Periodontology, College of Dentistry, University of Florida, Gainesville, FL, USA.
University of Florida · USUniversity of Kentucky · US

Funding

Mechanisms and Treatment Response of Aggressive Periodontitis in ChildrenR01DE019456 · NIDCR · UNIVERSITY OF FLORIDA · PI AUKHIL, IKRAMUDDIN, SHADDOX, LUCIANA MACCHION · 2009 to 2018
$5.6M
NIDCR NIH HHS R01 DE019456
6 · The paper itself

Abstract

aimThe purpose of this study was to test for the role of the P2X7 receptor in localized aggressive periodontitis (LAP).

methodsPeripheral blood was obtained from 95 subjects with LAP and 76 healthy unrelated controls (HUCs). Three P2RX7 single-nucleotide polymorphisms (rs1718119, rs2230911, and rs3751143) were genotyped from these subjects, and their peripheral blood samples were stimulated with lipopolysaccharide (LPS) from Escherichia coli and tested for inflammatory markers. The 3 P2RX7 single-nucleotide polymorphisms were in found to be in perfect linkage disequilibrium, and a total of 4 haplotypes and 9 diplotypes were identified among all subjects. For both subject populations, the 9 diplotypes were grouped into 4 functional groups and tested for association with subject inflammatory response. To specifically study the effects of extrinsic activation of the P2X7 receptor in LAP, peripheral blood samples from were stimulated under 3 treatments: LPS, LPS + ATP, and LPS +ATP+ P2X7 selective inhibitor. The effects of these treatments on P2X7 receptor activity were measured through Luminex protein assay. Last, to test whether receptor stimulation was related to P2RX7 expression, relative mRNA levels of P2RX7 were quantified with real-time quantitative polymerase chain reaction.

resultsSeveral associations between the P2RX7 diplotypes and LPS-stimulated blood chemokine/cytokine levels were found between the LAP and HUC populations (P < 0.05). P2X7 activation resulted in statistically significant differences in IL-1β and IL-12p40 concentrations for both subject populations. The relative P2RX7 mRNA levels increased significantly after addition of its inhibitor for both LAP and HUC populations.

conclusionsThis study detected an association between P2RX7 functional diplotypes and in vitro immune response of whole blood from subjects with LAP. In addition, we found that inhibition of the activated P2X7 receptor leads to increased P2RX7 mRNA levels, suggesting a feedback loop ( ClinicalTrials.gov NCT01330719). KNOWLEDGE TRANSFER STATEMENT: The results of this study suggest that P2RX7 functional diplotypes are associated with LAP and their in vitro immune response to bacteria. Ongoing studies to uncover the mechanistic link between P2RX7 and LAP phenotypes could lead to the development of preventive approaches for susceptible subjects.

Indexed as

Aggressive PeriodontitisGenotypeHaplotypesHumansLinkage DisequilibriumPolymorphism, Single Nucleotidecytokine(s)geneticsinflammationmolecular geneticsperiodontal diseasessingle nucleotide polymorphism

Identifiers

PMID31319038
PMCPMC6749795
OpenAlexW2960293491

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.