ArticleMolecular biology of the cell2019
Myosin IIA-mediated forces regulate multicellular integrity during vascular sprouting.
Article in Molecular biology of the cell, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 35 citations in OpenAlex.
- Active Microrheology Reveals Distinct ECM Mechanical Signatures Induced by Stromal Cells of Different Tissue Origins during Vascular Morphogenesis.ACS biomaterials science & engineering · 2026Article
- Mechanobiology of solid tumor vasculature: implications for tumor progression.Cancer metastasis reviews · 2026Review
- Investigation of mechanical forces during multicellular early angiogenic sprouting by three-dimensional traction force microscopy in hydrogel matrices.Nature protocols · 2026Review
- Guidance for 3D traction force microscopy today and in the next decade.Nature methods · 2026Review
- Article
- Neurogranin-MYH9 interaction regulates cytoskeletal remodeling in cerebral vasculature.Fluids and barriers of the CNS · 2025Article
- Paracingulin controls the junctional accumulation of nonmuscle myosin-2 in endothelial cells in vivo.microPublication biology · 2025Article
- Bioengineering vascularization.Development (Cambridge, England) · 2024Review
- Force-mediated recruitment and reprogramming of healthy endothelial cells drive vascular lesion growth.Nature communications · 2024Article
- YAP/TAZ drives Notch and angiogenesis mechanoregulation in silico.NPJ systems biology and applications · 2024Article
- Hydrogels with tunable mechanical plasticity regulate endothelial cell outgrowth in vasculogenesis and angiogenesis.Nature communications · 2023Article
- External mechanical loading overrules cell-cell mechanical communication in sprouting angiogenesis during early bone regeneration.PLoS computational biology · 2023Article
- 'Chip'-ing away at morphogenesis - application of organ-on-chip technologies to study tissue morphogenesis.Journal of cell science · 2023Article
- Stiffening Matrix Induces Age-Mediated Microvascular Phenotype Through Increased Cell Contractility and Destabilization of Adherens Junctions.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Bioprinted microvasculature: progressing from structure to function.Biofabrication · 2022Review
- Stromal cell identity modulates vascular morphogenesis in a microvasculature-on-a-chip platform.Lab on a chip · 2021Article
- Dynamic Endothelial Stalk Cell-Matrix Interactions Regulate Angiogenic Sprout Diameter.Frontiers in bioengineering and biotechnology · 2021Article
- Active biomaterials for mechanobiology.Biomaterials · 2021Review
- Hydrogel Network Dynamics Regulate Vascular Morphogenesis.Cell stem cell · 2020Article
- Nonmuscle myosin 2 regulates cortical stability during sprouting angiogenesis.Molecular biology of the cell · 2020Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
Abstract
Angiogenic sprouting is a critical process involved in vascular network formation within tissues. During sprouting, tip cells and ensuing stalk cells migrate collectively into the extracellular matrix while preserving cell-cell junctions, forming patent structures that support blood flow. Although several signaling pathways have been identified as controlling sprouting, it remains unclear to what extent this process is mechanoregulated. To address this question, we investigated the role of cellular contractility in sprout morphogenesis, using a biomimetic model of angiogenesis. Three-dimensional maps of mechanical deformations generated by sprouts revealed that mainly leader cells, not stalk cells, exert contractile forces on the surrounding matrix. Surprisingly, inhibiting cellular contractility with blebbistatin did not affect the extent of cellular invasion but resulted in cell-cell dissociation primarily between tip and stalk cells. Closer examination of cell-cell junctions revealed that blebbistatin impaired adherens-junction organization, particularly between tip and stalk cells. Using CRISPR/Cas9-mediated gene editing, we further identified NMIIA as the major isoform responsible for regulating multicellularity and cell contractility during sprouting. Together, these studies reveal a critical role for NMIIA-mediated contractile forces in maintaining multicellularity during sprouting and highlight the central role of forces in regulating cell-cell adhesions during collective motility.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.