Evidence map›Paper›PMID 31317494›Full record

ReviewAdvances in experimental medicine and biology2019

Single Virion Tracking Microscopy for the Study of Virus Entry Processes in Live Cells and Biomimetic Platforms.

Lakshmi Nathan, Susan Daniel

Abstract readReview
In one paragraph

Review in Advances in experimental medicine and biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

  1. Article
  2. From Biophysics to Biomedical Physics.ACS bio & med chem Au · 2025
    Review
  3. Article
  4. Article
  5. Making Rift Valley Fever Viral Particles Fluorescent.Methods in molecular biology (Clifton, N.J.) · 2024
    Article
  6. Review
  7. Article
  8. Review
  9. Article
  10. Review
  11. Review
  12. Article
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Lakshmi NathanRobert Frederick Smith School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY, USA. ln258@cornell.edu.
Susan DanielRobert Frederick Smith School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY, USA. sd386@cornell.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The most widely-used assays for studying viral entry, including infectivity, cofloatation, and cell-cell fusion assays, yield functional information but provide low resolution of individual entry steps. Structural characterization provides high-resolution conformational information, but on its own is unable to address the functional significance of these conformations. Single virion tracking microscopy techniques provide more detail on the intermediate entry steps than infection assays and more functional information than structural methods, bridging the gap between these methods. In addition, single virion approaches also provide dynamic information about the kinetics of entry processes. This chapter reviews single virion tracking techniques and describes how they can be applied to study specific virus entry steps. These techniques provide information complementary to traditional ensemble approaches. Single virion techniques may either probe virion behavior in live cells or in biomimetic platforms. Synthesizing information from ensemble, structural, and single virion techniques ultimately yields a more complete understanding of the viral entry process than can be achieved by any single method alone.

Indexed as

BiomimeticsCellsMicroscopyVirus InternalizationVirologyBiomimeticsEnveloped virusFluorescence microscopyImagingInfectionLipid bilayerLive cellsMembrane fusionNon-enveloped virusSingle virus particleTrackingVirion trackingVirus entry

Identifiers

PMID31317494
PMCPMC7122913

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.