ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2019
Next-generation sequencing-based bulked segregant analysis for QTL mapping in the heterozygous species Brassica rapa.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
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- A key gene for vernalization-independent bolting under extra-long days in biennial sugar beet.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Reverse BSA-QTLseq: A new genotype-driven bioinformatics approach for simultaneous trait mapping.Plant communications · 2026Article
- FScientific reports · 2025Article
- DNAMarkMaker: streamlining ARMS and CAPS marker development from resequencing data with NGS short reads.Breeding science · 2024Article
- QTL-Seq identified a genomic region on chromosome 1 for soil-salinity tolerance in FFrontiers in plant science · 2024Article
- Non-vernalization requirement for flowering in Brassica rapa conferred by a dominant allele of FLOWERING LOCUS T.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Mutations in RZF1, a zinc-finger protein, reduce magnesium uptake in roots and translocation to shoots in rice.Plant physiology · 2023Article
- Upregulation of tandem duplicated BoFLC1 genes is associated with the non-flowering trait in Brassica oleracea var. capitata.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Mapping-by-Sequencing Reveals Genomic Regions Associated with Seed Quality Parameters inGenes · 2022Article
- Polyploid QTL-seq towards rapid development of tightly linked DNA markers for potato and sweetpotato breeding through whole-genome resequencing.Plant biotechnology journal · 2021Article
- Sat-BSA: an NGS-based method using localBreeding science · 2021Article
- Recent advances in Cannabis sativa genomics research.The New phytologist · 2021Review
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Authors and funding
12 authors.
Funding
Abstract
key messageAn improved protocol of QTL-seq, an NGS-based method for bulked segregant analysis we previously developed in rice, allowed successful mapping of QTLs of interest in the highly heterozygous genome of B. rapa, demonstrating the power of this elegant method for genetic analyses in heterozygous species of economic importance. Recent advances in next-generation sequencing (NGS) and the various NGS-based methods developed for rapidly identifying candidate genes of interest have accelerated genetic analysis mainly in the model plants rice and Arabidopsis. Brassica rapa includes several economically important crops such as Chinese cabbage, turnip and various leafy vegetables. The application of NGS-based approaches for the analysis of B. rapa has been limited mainly due to its highly heterozygous genome and poor quality of the reference genome sequence currently available for this species. In this study, we have improved QTL-seq, a method for NGS-based bulked segregant analysis we previously developed in rice, extending its applicability for accelerating the genetic analysis and molecular breeding of B. rapa. Addition of new filters to the original QTL-seq pipeline allowed removal of spurious single-nucleotide polymorphisms caused by alignment/sequencing errors and variability between parents, significantly improving accuracy of the analysis. As proof of principle, we successfully applied the new approach to identify candidate genomic regions controlling flowering and trichome formation using segregating F2 progeny obtained from crosses made between cultivars of B. rapa showing contrasting phenotypes for these traits. We strongly believe that the improved QTL-seq method reported here will extend the applicability of NGS-based genetic analysis not only to B. rapa but also to other plant species of economic importance with heterozygous genomes.
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