ArticlePlant methods2019
Refinement of a clearing protocol to study crassinucellate ovules of the sugar beet (
Article in Plant methods, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Remote sensing estimation of sugar beet SPAD based on un-manned aerial vehicle multispectral imagery.PloS one · 2024Article
- Origin, genetic structure and evolutionary potential of the natural hybrid Ranunculus circinatus × R. fluitans.Scientific reports · 2023Article
- Production of Gynogenic Plants of Red Beet (Plants (Basel, Switzerland) · 2021Article
- Vibratome Sectioning and Clearing for Easing Studies of Cassava Embryo Formation.Frontiers in plant science · 2020Article
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5 authors.
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Abstract
backgroundClearing methods allow relatively quick processing of plant material and examination of cellular structures by rendering tissues and organs translucent. They have been adapted for plant embryology, primarily to study ovule development, megasporogenesis, megagametogenesis and embryogenesis. Such clearing methods overcome several disadvantages of the conventional embedding-sectioning techniques that are arduous and time-consuming. Although numerous protocols with different clearing solutions have been described, there have been no reports to date proposing a reliable method to clear the crassinucellate ovules of the sugar beet (
resultsWe established a methyl salicylate-based protocol that significantly improved the transparency of the
conclusionsThe effectiveness of our method is due to the strategy combining various approaches at different stages of the procedure aiming at increasing the accessibility of the internal ovule structures to the clearing solution. The results of this study may be applied in sugar beet breeding programs, and it will provide a basis for further investigation of numerous aspects of the species' embryology. Moreover, that unique approach may be easily adapted to other species developing crassinucellate ovules.
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