ArticleFrontiers in neuroscience2019
Unbiased Analysis Method for Measurement of Red Blood Cell Size and Velocity With Laser Scanning Microscopy.
Article in Frontiers in neuroscience, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 21 citations in OpenAlex.
- Protocol for In Vivo Two-Photon FCS to Measure Nanocarrier Number and Flow Velocity in Mouse Cerebral Microvasculature.Bio-protocol · 2026Article
- Partitioning of dense RBC suspensions in single microfluidic bifurcations: role of cell deformability and bifurcation angle.Scientific reports · 2024Article
- Spatiotemporal analysis of blood plasma and blood cell flow fluctuations of cerebral microcirculation in anesthetized rats.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2023Article
- Microcapillary cell extrusion deposition with picolitre dispensing resolution.Bio-design and manufacturing · 2023Article
- Hematocrit skewness along sequential bifurcations within a microfluidic network induces significant changes in downstream red blood cell partitioning.Biomicrofluidics · 2022Article
- Quantitative Hemodynamic Measurements in Cortical Vessels Using Functional Ultrasound Imaging.Frontiers in neuroscience · 2022Article
- Measurement of Blood Velocity With Laser Scanning Microscopy: Modeling and Comparison of Line-Scan Image-Processing Algorithms.Frontiers in physiology · 2022Article
- Cross talk between endothelial and red blood cell glycocalyces via near-field flow.Biophysical journal · 2021Article
- A Permanent Window for Investigating Cancer Metastasis to the Lung.Journal of visualized experiments : JoVE · 2021Article
- Imaging and optogenetic modulation of vascular mural cells in the live brain.Nature protocols · 2021Article
- Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two-photon laser scanning microscopy is widely used to measure blood hemodynamics in brain blood vessels. Still, the algorithms used so far to extract red blood cell (RBC) size and velocity from line-scan acquisitions have ignored the extent to which scanning speed influences the measurements. Here, we used a theoretical approach that takes into account the velocity and direction of both scanning mirrors and RBCs during acquisition to provide an algorithm that measures the real RBC size and velocity. We validate our approach in brain vessels of anesthetized mice, and demonstrate that it corrects online measurement errors that can reach several 10s of percent as well as data previously acquired. To conclude, our analysis allows unbiased comparisons of blood hemodynamic parameters from brain capillaries and large vessels in control and pathological animal models.
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