ArticleScientific reports2019
Sperm-borne miR-216b modulates cell proliferation during early embryo development via K-RAS.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 61 citations in OpenAlex.
- In Vitro Sperm-Epididymosomes Interaction Immediately Before Fertilization Changes Sperm Fertility Potential.Andrology · 2026Article
- Epididymosome-Supplemented Extender Induces Changes in Morpho-Functional Traits and microRNA Levels of Post-Thaw Sperm and Improves Embryo Developmental Potential.Molecular reproduction and development · 2026Article
- Intraepididymal platelet-rich plasma improves semen cryoresistance via antioxidant, lipid and molecular modulation during the non-breeding season in rams.Veterinary research communications · 2026Article
- Normozoospermic seminal fluid extracellular vesicles could ameliorate adverse effects of freeze-thaw in oligoasthenoteratospermia men.Journal of assisted reproduction and genetics · 2025Article
- microRNAs for qPCR Normalization Under Morphofunctional Conditions in Bovine Sperm (Bos taurus).Molecular reproduction and development · 2025Article
- Age-related decline in behavior and reproductive health in male mice.Scientific reports · 2025Article
- Sperm-Derived CircRNA-1572 Regulates Embryogenesis and Zygotic Genome Activation by Targeting CCNB2 via Bta-miR-2478-L-2.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Sperm-borne small non-coding RNAs: potential functions and mechanisms as epigenetic carriers.Cell & bioscience · 2025Review
- Scans for Signatures of Selection in Genomes of Wagyu and Buryat Cattle Breeds Reveal Candidate Genes and Genetic Variants for Adaptive Phenotypes and Production Traits.Animals : an open access journal from MDPI · 2024Article
- Reproductive Ageing: Current insights and a potential role of NAD in the reproductive health of aging fathers and their children.Reproduction (Cambridge, England) · 2024Review
- Multi-omics data integration for the identification of biomarkers for bull fertility.PloS one · 2024Article
- THC and sperm: Impact on fertilization capability, pre-implantation in vitro development and epigenetic modifications.PloS one · 2024Article
- Harnessing male germline epigenomics for the genetic improvement in cattle.Journal of animal science and biotechnology · 2023Review
- Sperm-borne microRNA-34c regulates maternal mRNA degradation and preimplantation embryonic development in mice.Reproductive biology and endocrinology : RB&E · 2023Article
- Emerging evidence that the mammalian sperm epigenome serves as a template for embryo development.Nature communications · 2023Review
- Glucocorticoid exposure modifies the miRNA profile of sperm in the guinea pig: Implications for intergenerational transmission.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2023Article
- High-throughput sequencing reveals hub genes for human early embryonic development arrestFrontiers in physiology · 2023Article
- Normospermic Patients Infected WithPathogens & immunity · 2023Article
- Extracellular vesicles from seminal plasma to improve fertilizing capacity of bulls.Reproduction & fertility · 2022Article
- Integrated analysis of expression profiles with meat quality traits in cattle.Scientific reports · 2022Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Semen fertilizing potential is dependent upon the morphological, functional and molecular attributes of sperm. Sperm microRNAs (miRNAs) were recently shown to hold promise regarding their association with different fertility phenotypes. However, their role in fertility regulation remains to be determined. We postulated that sperm miRNAs might regulate early embryonic development. From this perspective, sperm quality and 380 sperm miRNAs were investigated in frozen-thawed semen from high (HF; 54.3 ± 1.0% pregnancy rate) and low (LF; 41.5 ± 2.3%) fertility bulls. Out of nine miRNAs that showed different levels in sperm cells, miR-216b was present at lower levels in HF sperm cells and zygotes. Among miR-216b target genes (K-RAS, BECN1 and JUN), K-RAS, related to cell proliferation, revealed a higher level in HF two-cell embryos. First cleavage rate, blastocyst cell number and division number were also higher in HF. In addition, by using a model based on polyspermy embryos, we demonstrated an increase in miR-216b levels in zygotes associated with sperm cell entry. Our results shed light on a possible mechanism of paternal contribution involving sperm-borne miR-216b that modulates levels of miR-216b in zygotes and K-RAS in two-cell embryos. This modulation might regulate early development by interfering with the first cleavage and blastocyst quality.
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Registered trials
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