ArticleScientific reports2019
Molecular contrast on phase-contrast microscope.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Differential Phase Contrast Imaging to Predict MSC Immune Function.Advanced healthcare materials · 2025Article
- Advanced vibrational microscopes for life science.Nature methods · 2025Review
- Ultrabroadband Optical Diffraction Tomography.ACS photonics · 2024Article
- A tutorial on optical photothermal infrared (O-PTIR) microscopy.APL photonics · 2024Article
- Vibrational imaging of metabolites for improved microbial cell strains.Journal of biomedical optics · 2024Review
- Monitoring the synthesis of neutral lipids in lipid droplets of living human cancer cells using two-color infrared photothermal microscopy.Chemical science · 2024Article
- Development of label-free light-controlled gene expression technologies using mid-IR and terahertz light.Frontiers in bioengineering and biotechnology · 2024Review
- Label-free mid-infrared photothermal live-cell imaging beyond video rate.Light, science & applications · 2023Article
- Video-rate mid-infrared photothermal imaging by single-pulse photothermal detection per pixel.Science advances · 2023Article
- Ultrafast Widefield Mid-Infrared Photothermal Heterodyne Imaging.Analytical chemistry · 2022Article
- Wide-Field Mid-Infrared Hyperspectral Imaging by Snapshot Phase Contrast Measurement of Optothermal Excitation.Analytical chemistry · 2021Article
- Background-Suppressed High-Throughput Mid-Infrared Photothermal Microscopy via Pupil Engineering.ACS photonics · 2021Article
- Bond-selective imaging by optically sensing the mid-infrared photothermal effect.Science advances · 2021Review
- Adaptive dynamic range shift (ADRIFT) quantitative phase imaging.Light, science & applications · 2021Article
- Symposium report: understanding biological systems with quantum science and technology.Biophysical reviews · 2020Article
- Article
- Bond-selective transient phase imaging via sensing of the infrared photothermal effect.Light, science & applications · 2019Article
- Microscopes in Conservative Dentistry and Endodontics Research.Journal of conservative dentistry : JCDArticle
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An optical microscope enables image-based findings and diagnosis on microscopic targets, which is indispensable in many scientific, industrial and medical settings. A standard benchtop microscope platform, equipped with e.g., bright-field and phase-contrast modes, is of importance and convenience for various users because the wide-field and label-free properties allow for morphological imaging without the need for specific sample preparation. However, these microscopes never have capability of acquiring molecular contrast in a label-free manner. Here, we develop a simple add-on optical unit, comprising of an amplitude-modulated mid-infrared semiconductor laser, that is attached to a standard microscope platform to deliver the additional molecular contrast of the specimen on top of its conventional microscopic image, based on the principle of photothermal effect. We attach this unit, termed molecular-contrast unit, to a standard phase-contrast microscope, and demonstrate high-speed label-free molecular-contrast phase-contrast imaging of silica-polystyrene microbeads mixture and molecular-vibrational spectroscopic imaging of HeLa cells. Our simple molecular-contrast unit can empower existing standard microscopes and deliver a convenient accessibility to the molecular world.
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