Evidence map›Paper›PMID 31315955›Full record

ArticleJournal of clinical microbiology2019

Metagenomic Next-Generation Sequencing of the 2014 Ebola Virus Disease Outbreak in the Democratic Republic of the Congo.

Tony Li, Placide Mbala-Kingebeni, Samia N Naccache, Julien Thézé, Jerome Bouquet, Scot Federman, Sneha Somasekar, Guixia Yu, Claudia Sanchez-San Martin, Asmeeta Achari and 10 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of clinical microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed, 1 pooled it
2.0field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 1 synthesis or guideline pooled it, 33 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Article
  4. Review
  5. Article
  6. The Case for Genomic Surveillance in Africa.Tropical medicine and infectious disease · 2025
    Review
  7. Review
  8. Article
  9. Article
  10. Review
  11. Article
  12. Article
  13. Review
  14. Article
  15. Article
  16. Review
  17. Article
  18. Viral genomics in Ebola virus research.Nature reviews. Microbiology · 2020
    Review
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors at 6 institutions in 3 countries.

Tony Li *Department of Laboratory Medicine, University of California, San Francisco, San Francisco, California, USA.
Placide Mbala-Kingebeni *Institut National de Recherche Biomédicale, Kinshasa, Democratic Republic of the Congo.
Samia N NaccacheDepartment of Laboratory Medicine, University of California, San Francisco, San Francisco, California, USA.
Julien ThézéDepartment of Zoology, University of Oxford, Oxford, United Kingdom.
Jerome BouquetDepartment of Laboratory Medicine, University of California, San Francisco, San Francisco, California, USA.
Scot FedermanDepartment of Laboratory Medicine, University of California, San Francisco, San Francisco, California, USA.
Sneha SomasekarDepartment of Laboratory Medicine, University of California, San Francisco, San Francisco, California, USA.
Guixia YuDepartment of Laboratory Medicine, University of California, San Francisco, San Francisco, California, USA.
Claudia Sanchez-San MartinDepartment of Laboratory Medicine, University of California, San Francisco, San Francisco, California, USA.
Asmeeta AchariDepartment of Laboratory Medicine, University of California, San Francisco, San Francisco, California, USA.
Bradley S SchneiderEtiologic, Inc., San Francisco, California, USA.
Anne W RimoinDepartment of Epidemiology, School of Public Health, University of California, Los Angeles, Los Angeles, California, USA.
Andrew RambautInstitute of Evolutionary Biology, University of Edinburgh, Edinburgh, United Kingdom.
Justus NsioMinistry of Public Health, Kinshasa, Democratic Republic of the Congo.
Prime MulembakaniInstitut National de Recherche Biomédicale, Kinshasa, Democratic Republic of the Congo.
Steve Ahuka-MundekeInstitut National de Recherche Biomédicale, Kinshasa, Democratic Republic of the Congo.
Jimmy KapetshiInstitut National de Recherche Biomédicale, Kinshasa, Democratic Republic of the Congo.
Oliver G PybusDepartment of Zoology, University of Oxford, Oxford, United Kingdom.
Jean-Jacques Muyembe-TamfumInstitut National de Recherche Biomédicale, Kinshasa, Democratic Republic of the Congo.
Charles Y ChiuDepartment of Laboratory Medicine, University of California, San Francisco, San Francisco, California, USA Charles.chiu@ucsf.edu.ORCID 0000-0003-2915-2094
University of California, San Francisco · USNational Institute of Biomedical Research · CDUniversity of Oxford · GBMinistry of Public Health · CDUniversity of California, Los Angeles · USUniversity of Edinburgh · GB

Funding

Pan-Genomic Approaches for Comprehensive Blood Screening of Novel or Emerging Infectious AgentsR01HL105704 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI CHIU, CHARLES YEN · 2011 to 2020
$5.9M
Real-Time Unbiased Pathogen Detection in Febrile Illnesses by Nanopore SequencingR33AI120977 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI CHIU, CHARLES YEN · 2018 to 2020
$1.6M
Real-Time Unbiased Pathogen Detection in Febrile Illnesses by Nanopore SequencingR21AI120977 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI CHIU, CHARLES YEN · 2016 to 2017
$436k
NHLBI NIH HHS R01 HL105704NIAID NIH HHS R21 AI120977NIAID NIH HHS R33 AI120977Wellcome Trust
6 · The paper itself

Abstract

We applied metagenomic next-generation sequencing (mNGS) to detect Zaire Ebola virus (EBOV) and other potential pathogens from whole-blood samples from 70 patients with suspected Ebola hemorrhagic fever during a 2014 outbreak in Boende, Democratic Republic of the Congo (DRC) and correlated these findings with clinical symptoms. Twenty of 31 patients (64.5%) tested in Kinshasa, DRC, were EBOV positive by quantitative reverse transcriptase PCR (qRT-PCR). Despite partial degradation of sample RNA during shipping and handling, mNGS followed by EBOV-specific capture probe enrichment in a U.S. genomics laboratory identified EBOV reads in 22 of 70 samples (31.4%) versus in 21 of 70 (30.0%) EBOV-positive samples by repeat qRT-PCR (overall concordance = 87.1%). Reads from

Indexed as

Disease OutbreaksAdultCoinfectionDemocratic Republic of the CongoEbolavirusFemaleHemorrhagic Fever, EbolaHigh-Throughput Nucleotide SequencingHumansInfantMaleMetagenomicsMiddle AgedYoung Adult2014 Boende outbreakcoinfectionEbola virusmolecular clock analysisnext-generation sequencingOrungo viruspathogen discoveryphylogenetic analysisviral genome assemblyviral metagenomics

Identifiers

PMID31315955
PMCPMC6711896
OpenAlexW2957319915

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.