ArticleInternational journal of molecular sciences2019
Near-Infrared Genetically Encoded Positive Calcium Indicator Based on GAF-FP Bacterial Phytochrome.
Article in International journal of molecular sciences, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- A sensitive soma-localized red fluorescent calcium indicator for in vivo imaging of neuronal populations at single-cell resolution.PLoS biology · 2025Article
- Quantitative approaches for studying G protein-coupled receptor signalling and pharmacology.Journal of cell science · 2025Review
- Two-Photon Microscopy to Measure Calcium Signaling in the Living Brain.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Controlling the sound of light: photoswitching optoacoustic imaging.Nature methods · 2024Review
- Genetically encoded green fluorescent sensor for probing sulfate transport activity of solute carrier family 26 member a2 (Slc26a2) protein.Communications biology · 2024Article
- Fluorescent Protein-Based Sensors for Detecting Essential Metal Ions across the Tree of Life.ACS sensors · 2024Review
- Zinc-Induced Fluorescence Turn-On in Native and Mutant Phycoerythrobilin-Binding Orange Fluorescent Proteins.Biochemistry · 2023Article
- Functional imaging-guided cell selection for evolving genetically encoded fluorescent indicators.Cell reports methods · 2023Article
- Article
- A genetically encoded far-red fluorescent calcium ion biosensor derived from a biliverdin-binding protein.Protein science : a publication of the Protein Society · 2022Article
- LSSmScarlet2 and LSSmScarlet3, Chemically Stable Genetically Encoded Red Fluorescent Proteins with a Large Stokes' Shift.International journal of molecular sciences · 2022Article
- Dual-expression system for blue fluorescent protein optimization.Scientific reports · 2022Article
- Genetically encoded photo-switchable molecular sensors for optoacoustic and super-resolution imaging.Nature biotechnology · 2022Article
- The Red Edge: Bilin-Binding Photoreceptors as Optogenetic Tools and Fluorescence Reporters.Chemical reviews · 2021Review
- LSSmScarlet, dCyRFP2s, dCyOFP2s and CRISPRed2s, Genetically Encoded Red Fluorescent Proteins with a Large Stokes Shift.International journal of molecular sciences · 2021Article
- Near-infrared and far-red genetically encoded indicators of neuronal activity.Journal of neuroscience methods · 2021Article
- Optimizing Calcium Detection Methods in Animal Systems: A Sandbox for Synthetic Biology.Biomolecules · 2021Review
- The design and evolution of fluorescent protein-based sensors for monoatomic ions in biology.Protein engineering, design & selection : PEDS · 2021Review
- Emergent Biosensing Technologies Based on Fluorescence Spectroscopy and Surface Plasmon Resonance.Sensors (Basel, Switzerland) · 2021Review
- Photoacoustic Neuroimaging - Perspectives on a Maturing Imaging Technique and its Applications in Neuroscience.Frontiers in neuroscience · 2021Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
A variety of genetically encoded calcium indicators are currently available for visualization of calcium dynamics in cultured cells and in vivo. Only one of them, called NIR-GECO1, exhibits fluorescence in the near-infrared region of the spectrum. NIR-GECO1 is engineered based on the near-infrared fluorescent protein mIFP derived from bacterial phytochromes. However, NIR-GECO1 has an inverted response to calcium ions and its excitation spectrum is not optimal for the commonly used 640 nm lasers. Using small near-infrared bacterial phytochrome GAF-FP and calmodulin/M13-peptide pair, we developed a near-infrared calcium indicator called GAF-CaMP2. In vitro, GAF-CaMP2 showed a positive response of 78% and high affinity (K
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