ArticleJournal of virological methods2019
A strand-specific real-time quantitative RT-PCR assay for distinguishing the genomic and antigenomic RNAs of Rift Valley fever phlebovirus.
Article in Journal of virological methods, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed.
- Impact of cell type and species on RNA replication kinetics of Seoul virus.The Journal of general virology · 2025Article
- Five novel RNA viruses of the invasive big-headed ant (Pheidole megacephala).Archives of virology · 2025Article
- Novel strand-specific qPCR assay elucidates differences in viral replication kinetics between different strains of avian reovirus.Microbiology spectrum · 2025Article
- Expanding the evidence for cross-species viral transmission from trophic interactions of parasitoid wasps and their hosts.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Numb-associated kinases regulate sandfly-borne Toscana virus entry.Emerging microbes & infections · 2024Article
- N6-methyladenosine RNA modification promotes Severe Fever with Thrombocytopenia Syndrome Virus infection.PLoS pathogens · 2024Article
- Monoclonal Antibodies for Rift Valley Fever Virus Nucleocapsid: Application in IgG/IgM ELISA for Sero-Diagnosis.Pathogens (Basel, Switzerland) · 2024Article
- CK1 and PP1 regulate Rift Valley fever virus genome replication through L protein phosphorylation.Antiviral research · 2024Article
- Genome-wide view and characterization of natural antisense transcripts in Cannabis Sativa L.Plant molecular biology · 2024Article
- The genome of a bunyavirus cannot be defined at the level of the viral particle but only at the scale of the viral population.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Review
- Mechanistic insight into the efficient packaging of antigenomic S RNA into Rift Valley fever virus particles.Frontiers in cellular and infection microbiology · 2023Article
- Development and Validation of Rapid Colorimetric Reverse Transcription Loop-Mediated Isothermal Amplification for Detection of Rift Valley Fever Virus.Advances in virology · 2023Article
- Article
- Rift Valley fever virus 78kDa envelope protein attenuates virus replication in macrophage-derived cell lines and viral virulence in mice.PLoS neglected tropical diseases · 2021Article
- Characterization of the Molecular Interactions That Govern the Packaging of Viral RNA Segments into Rift Valley Fever Phlebovirus Particles.Journal of virology · 2021Article
- The Severe Fever with Thrombocytopenia Syndrome Virus NSs Protein Interacts with CDK1 To Induce GJournal of virology · 2020Article
- Development of a Visible Reverse Transcription-Loop-Mediated Isothermal Amplification Assay for the Detection of Rift Valley Fever Virus.Frontiers in microbiology · 2020Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Rift Valley Fever phlebovirus (RVFV), genus Phlebovirus, family Phenuiviridae, order Bunyavirales, has a single-stranded, negative-sense RNA genome, consisting of L, M and S segments. Here, we report the establishment of a strand-specific, quantitative reverse transcription (RT)-PCR assay system that can selectively distinguish between the genomic and antigenomic RNAs of each of the three viral RNA segments produced in RVFV-infected cells. To circumvent the obstacle of primer-independent cDNA synthesis during RT, we used a tagged, strand-specific RT primer, carrying a non-viral 'tag' sequence at the 5' end, which ensured the strand-specificity through the selective amplification of only the tagged cDNA in the real-time PCR assay. We used this assay system to examine the kinetics of intracellular accumulation of genomic and antigenomic viral RNAs in mammalian cells infected with the MP-12 strain of RVFV. The genomic RNA copy numbers, for all three viral RNA segments, were higher than that of their corresponding antigenomic RNAs throughout the time-course of infection, with a notable exception, wherein the M segment genomic and antigenomic RNAs exhibited similar copy numbers at specific times post-infection. Overall, this assay system could be a useful tool to gain an insight into the mechanisms of RNA replication and packaging in RVFV.
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