Evidence map›Paper›PMID 31314059›Full record

ArticleAmerican journal of clinical pathology2019

Comparison of Two Commercial Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) Systems for Identification of Nontuberculous Mycobacteria.

Barbara A Brown-Elliott, Thomas R Fritsche, Brooke J Olson, Sruthi Vasireddy, Ravikiran Vasireddy, Elena Iakhiaeva, Diana Alame, Richard J Wallace, John A Branda

Abstract readComparative Study
In one paragraph

Article in American journal of clinical pathology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed, 2 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 2 syntheses or guidelines pooled it.

  1. Pooled it
  2. Diagnostic performance of MALDI-TOF MS for non-tuberculous mycobacteria: A systematic review and meta-analysis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026
    Pooled it
  3. Article
  4. [Microbiological Characteristics of Extrapulmonary Nontuberculous Mycobacterial Infections in a Tertiary Hospital].Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2026
    Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Diagnostic and Therapeutic Challenges in DisseminatedOpen forum infectious diseases · 2023
    Article
  13. Diagnostic test accuracy of an automated device for the MALDI target preparation for microbial identification.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2023
    Article
  14. Article
  15. Article
  16. Review
  17. Article
  18. Article
  19. Host Immune Response and Novel Diagnostic Approach to NTM Infections.International journal of molecular sciences · 2020
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Barbara A Brown-ElliottThe University of Texas Health Science Center at Tyler.
Thomas R FritscheMarshfield Clinic Health System, Marshfield, WI.
Brooke J OlsonMarshfield Clinic Health System, Marshfield, WI.
Sruthi VasireddyThe University of Texas Health Science Center at Tyler.
Ravikiran VasireddyThe University of Texas Health Science Center at Tyler.
Elena IakhiaevaThe University of Texas Health Science Center at Tyler.
Diana AlameThomas Jefferson University, Philadelphia, PA.
Richard J WallaceThe University of Texas Health Science Center at Tyler.
John A BrandaMassachusetts General Hospital, Boston.

Funding

The Harvard Clinical and Translational Science CenterUL1TR002541 · NCATS · HARVARD MEDICAL SCHOOL · PI NADLER, LEE MARSHALL · 2018 to 2022
$93.0M
NCATS NIH HHS UL1 TR002541
6 · The paper itself

Abstract

objectivesThis multicenter study's aim was to assess the performance of two commercially available matrix-assisted laser desorption/ionization time of flight mass spectrometry systems in identifying a challenge collection of clinically relevant nontuberculous mycobacteria (NTM).

methodsNTM clinical isolates (n = 244) belonging to 23 species/subspecies were identified by gene sequencing and analyzed using Bruker Biotyper with Mycobacterial Library v5.0.0 and bioMérieux VITEK MS with v3.0 database.

resultsUsing the Bruker or bioMérieux systems, 92% and 95% of NTM strains, respectively, were identified at least to the complex/group level; 62% and 57%, respectively, were identified to the highest taxonomic level. Differentiation between members of Mycobacterium abscessus, M fortuitum, M mucogenicum, M avium, and M terrae complexes/groups was problematic for both systems, as was identification of M chelonae for the Bruker system.

conclusionsBoth systems identified most NTM isolates to the group/complex level, and many to the highest taxonomic level. Performance was comparable.

Indexed as

Diagnostic Tests, RoutineHumansNontuberculous MycobacteriaSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationbioMérieux VITEK MSBruker BiotyperComparisonIdentificationMALDI-TOFMass spectrometryNontuberculous mycobacteria

Identifiers

PMID31314059
PMCPMC6733354

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.