ArticleJournal of industrial microbiology & biotechnology2019
Increasing L-threonine production in Escherichia coli by overexpressing the gene cluster phaCAB.
Article in Journal of industrial microbiology & biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 28 citations in OpenAlex.
- Efficient production of glycine from glucose via a two-stage biosynthetic strategy.Microbial cell factories · 2026Article
- Reducing the sensitivity of Halomonas sp. to oxygen availability through adaptive laboratory evolution.Microbial cell factories · 2026Article
- Biological characterization of lipoic acid- and heme-dependent Escherichia coli small colony variants isolated from sheep in Xinjiang, China.Veterinary research communications · 2024Article
- Recent Advances in Metabolic Engineering for the Biosynthesis of Phosphoenol Pyruvate-Oxaloacetate-Pyruvate-Derived Amino Acids.Molecules (Basel, Switzerland) · 2024Review
- Engineering of increased L-Threonine production in bacteria by combinatorial cloning and machine learning.Metabolic engineering communications · 2023Article
- Construction of an Escherichia coli Strain Lacking Fimbriae by Deleting 64 Genes and Its Application for Efficient Production of Poly(3-Hydroxybutyrate) and l-Threonine.Applied and environmental microbiology · 2021Article
- Enhanced ascomycin production in Streptomyces hygroscopicus var. ascomyceticus by employing polyhydroxybutyrate as an intracellular carbon reservoir and optimizing carbon addition.Microbial cell factories · 2021Article
- Improving L-threonine production in Escherichia coli by elimination of transporters ProP and ProVWX.Microbial cell factories · 2021Article
- Metabolic Detoxification of 2-Oxobutyrate by RemodelingMetabolites · 2021Article
- Metabolic Engineering Design Strategies for Increasing Acetyl-CoA Flux.Metabolites · 2020Review
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
L-Threonine is an important branched-chain amino acid and could be applied in feed, drugs, and food. In this study, L-threonine production in an L-threonine-producing Escherichia coli strain TWF001 was significantly increased by overexpressing the gene cluster phaCAB from Ralstonia eutropha. TWF001/pFW01-phaCAB could produce 96.4-g/L L-threonine in 3-L fermenter and 133.5-g/L L-threonine in 10-L fermenter, respectively. In addition, TWF001/pFW01-phaCAB produced 216% more acetyl-CoA, 43% more malate, and much less acetate than the vector control TWF001/pFW01, and meanwhile, TWF001/pFW01-phaCAB produced poly-3-hydroxybutyrate, while TWF001/pFW01 did not. Transcription analysis showed that the key genes in the L-threonine biosynthetic pathway were up-regulated, the genes relevant to the acetate formation were down-regulated, and the gene acs encoding the enzyme which converts acetate to acetyl-CoA was up-regulated. The results suggested that overexpression of the gene cluster phaCAB in E. coli benefits the enhancement of L-threonine production.
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Registered trials
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