ArticleBioMed research international2019
MicroRNA Profiling of Exosomes Derived from Red Blood Cell Units: Implications in Transfusion-Related Immunomodulation.
Article in BioMed research international, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- ABCA1-Labeled Exosomes in Serum Contain Higher MicroRNA-193b Levels in Alzheimer's Disease.BioMed research international · 2021Trial
- Extracellular vesicles for next-gen therapeutics and drug delivery.Molecular biomedicine · 2026Review
- Red blood cells could protect miRNAs from degradation or loss thanks to Argonaute 2 binding.FEBS open bio · 2025Article
- Impact of intraoperative packed red blood cell transfusion volume on prognosis in CRS: a propensity-matched study.Frontiers in surgery · 2025Article
- Red blood cell extracellular vesicles: new frontiers in hematological biomarker discovery.Frontiers in medicine · 2025Review
- New and emerging technologies for pretransfusion blood quality assessment: A state-of-the-art review.Transfusion · 2024Review
- Perioperative Blood Transfusions and Cancer Progression: A Narrative Review.Current oncology reports · 2024Review
- Apoptotic vesicles derived from human red blood cells promote bone regeneration via carbonic anhydrase 1.Cell proliferation · 2024Article
- Red Blood Cell-Derived Extracellular Vesicles: An Overview of Current Research Progress, Challenges, and Opportunities.Biomedicines · 2023Review
- The effect of exosomes released from apheresis platelet concentrates under the impact of gamma irradiation and storage time upon platelet aggregation and hemostasis.Blood transfusion = Trasfusione del sangue · 2023Article
- Differential Effect of Extracellular Vesicles Derived fromInternational journal of molecular sciences · 2023Article
- Extracellular Vesicles as New Players in Drug Delivery: A Focus on Red Blood Cells-Derived EVs.Pharmaceutics · 2023Review
- Advances in Microfluidics for Single Red Blood Cell Analysis.Biosensors · 2023Review
- Identification of hsa-miR-619-5p and hsa-miR-4454 in plasma-derived exosomes as a potential biomarker for lung adenocarcinoma.Frontiers in genetics · 2023Article
- MicroRNAs as Quality Assessment Tool in Stored Packed Red Blood Cell in Blood Banks.Journal of blood medicine · 2023Review
- Roles and Applications of Red Blood Cell-Derived Extracellular Vesicles in Health and Diseases.International journal of molecular sciences · 2022Review
- Characterization of Microfragmented Adipose Tissue Architecture, Mesenchymal Stromal Cell Content and Release of Paracrine Mediators.Journal of clinical medicine · 2022Article
- Blood cell-derived extracellular vesicles: diagnostic biomarkers and smart delivery systems.Bioengineered · 2021Article
- Osteoblast Differentiation and Signaling: Established Concepts and Emerging Topics.International journal of molecular sciences · 2021Review
- The accumulation of exosome-associated microRNA-1246 and microRNA-150-3p in human red blood cell suspensions.Journal of translational medicine · 2021Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo elucidate the microRNAs existent in exosomes derived from stored red blood cell (RBC) unit and their potential function. MATERIALS AND
methodsExosomes were isolated from the supernatant derived from stored RBC units by sequential centrifugation. Isolated exosomes were characterized by TEM (transmission electron microscopy), western blotting, and DLS (dynamic light scattering). MicroRNA (miRNA) microarray was performed to detect the expression of miRNAs in 3 exosome samples. Results revealed miRNAs that were simultaneously expressed in the 3 exosome samples and were previously reported to exist in mature RBCs. Functions and potential pathways of some detected miRNAs were illustrated by bioinformatic analysis. Validation of the top 3 abundant miRNAs was carried out by qRT-PCR (quantitative reverse transcription-polymerase chain reaction).
resultsTEM and DLS revealed the mean size of the exosomes (RBC-derived) as 64.08 nm. These exosomes exhibited higher abundance of short RNA than the long RNA. 78 miRNAs were simultaneously detected in 3 exosome samples and mature RBCs. Several biological processes might be impacted by these miRNAs, through their target gene(s) enriched in a particular signalling pathway. The top 3 (abundant) miRNAs detected were as follows: miR-125b-5p, miR-4454, and miR-451a. qRT-PCR revealed higher abundance of miR-451a than others. Only miR-4454 and miR-451a abundance tended to increase with increasing storage time.
conclusionExosomes derived from stored RBC units possessed multiple miRNAs and, hence, could serve various functions. The function of exosomes (RBC-derived) might be implemented partly by the predominantly enriched miR-451a.
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