ArticleScientific reports2019
Glucose, adrenaline and palmitate antagonistically regulate insulin and glucagon secretion in human pseudoislets.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 23 citations in OpenAlex.
- Subaqueous acoustic pressure system based one day heterotypic pseudo-islet spheroid formation with adipose derived stem cells for graft survival-related function enhancement.Bioactive materials · 2025Article
- Scaffold-free endocrine tissue engineering: role of islet organization and implications in type 1 diabetes.BMC endocrine disorders · 2025Review
- Fluorescein-based sensors to purify human α-cells for functional and transcriptomic analyses.eLife · 2023Article
- Renewable Human Cell Model for Type 1 Diabetes Research: EndoC-Journal of diabetes research · 2023Article
- Sphingolipid subtypes differentially control proinsulin processing and systemic glucose homeostasis.Nature cell biology · 2023Article
- Effects of adrenergic-stimulated lipolysis and cytokine production on in vitro mouse adipose tissue-islet interactions.Scientific reports · 2022Article
- Article
- Microwell culture platform maintains viability and mass of human pancreatic islets.Frontiers in endocrinology · 2022Article
- Review
- Islet sympathetic innervation and islet neuropathology in patients with type 1 diabetes.Scientific reports · 2021Article
- FFA2-, but not FFA3-agonists inhibit GSIS of human pseudoislets: a comparative study with mouse islets and rat INS-1E cells.Scientific reports · 2020Article
- Article
- Functional analysis of islet cells in vitro, in situ, and in vivo.Seminars in cell & developmental biology · 2020Review
- The challenge of correctly reporting hormones content and secretion in isolated human islets.Molecular metabolism · 2019Review
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Isolated human islets do not always meet the quality standards required for transplant survival and reliable functional in vitro studies. The formation of pseudoislets, i.e. the reaggregation of a defined number of islet cells after dissociation, improves insulin secretion. We present a simple method of pseudoislet formation from human islet cells and assess the transcriptome and function of isolated human islets and pseudoislets from the same organ donors. Following pseudoislet formation, insulin content/DNA and mRNA/RPS13 resembled that of islets. In pseudoislets, glucose-stimulated insulin secretion (GSIS) was significantly higher (8-13-fold) than in islets (2-4-fold). GSIS of pseudoislets was partly inhibited by the glucagon-like peptide-1 receptor (GLP-1R) antagonist exendin-9. The stimulatory effects of palmitate and forskolin at 12 mM glucose were also significantly higher in pseudoislets than in islets. Further analysis of pseudoislets revealed that regulation of secretion and insulin and glucagon content was maintained over a longer culture period (6-14 d). While adrenaline inhibited GSIS, adrenaline together with palmitate stimulated glucagon secretion 2-fold at low glucose, an effect suppressed by high glucose. Transcriptome analysis revealed that, unlike islets, pseudoislets were deprived of exocrine and endothelial cells. In conclusion, pseudoislet formation restores functional integrity of human islet cells and allows long-term in vitro testing.
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