ArticlePloS one2019
Overexpression of the Lolium perenne L. delta1-pyrroline 5-carboxylate synthase (LpP5CS) gene results in morphological alterations and salinity tolerance in switchgrass (Panicum virgatum L.).
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 23 citations in OpenAlex.
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- Anthocyanin biosynthesis enhances alkaline stress tolerance by upregulating antioxidant machinery and modulating ROS levels in three red landraces of rice.BMC plant biology · 2025Article
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- Proline Tagging for Stress Tolerance in Plants.International journal of genomics · 2025Review
- The Biostimulant Effect of Hydroalcoholic Extracts ofPlants (Basel, Switzerland) · 2022Article
- Tissue Culture and Somatic Embryogenesis in Warm-Season Grasses-Current Status and Its Applications: A Review.Plants (Basel, Switzerland) · 2022Review
- Expression of Arabidopsis Ornithine Aminotransferase (AtOAT) encoded gene enhances multiple abiotic stress tolerances in wheat.Plant cell reports · 2021Article
- Metabolic engineering of osmoprotectants to elucidate the mechanism(s) of salt stress tolerance in crop plants.Planta · 2021Review
- Proline Biosynthesis Enzyme Genes Confer Salt Tolerance to Switchgrass (Frontiers in plant science · 2020Article
- Proline improves switchgrass growth and development by reduced lignin biosynthesis.Scientific reports · 2019Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In plants, Δ1-pyrroline- 5-carboxylate synthase (P5CS) is the rate-limiting enzyme in proline biosynthesis. In this study, we introduced the LpP5CS (Lolium perenne L.) gene into switchgrass by Agrobacterium-mediated transformation. The transgenic lines (TG) were classified into two groups based on their phenotypes and proline levels. The group I lines (TG4 and TG6) had relatively high proline levels and improved biomass yield. The group II lines (TG1 and TG2) showed low proline levels, severely delayed flowering, stunted growth and reduced biomass yield. Additionally, we used RNA-seq analysis to detect the most significant molecular changes, and we analyzed differentially expressed genes, such as flowering-related and CYP450 family genes. Moreover, the biomass yield, physiological parameters, and expression levels of reactive oxygen species scavenger-related genes under salt stress all indicated that the group I plants exhibited significantly increased salt tolerance compared with that of the control plants, in contrast to the group II plants. Thus, genetic improvement of switchgrass by overexpressing LpP5CS to increase proline levels is feasible for increasing plant stress tolerance.
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