ArticleNature methods2019
High-affinity free ubiquitin sensors for quantifying ubiquitin homeostasis and deubiquitination.
Article in Nature methods, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 30 citations in OpenAlex.
- A designed ubiquitin-binding protein selectively enriches and visualizes unanchored ubiquitin.Science advances · 2026Article
- Highly specific intracellular ubiquitination of a small molecule.Nature chemical biology · 2026Article
- Avidity-based biosensors for ubiquitylated PCNA reveal choreography of DNA damage bypass.Science advances · 2023Article
- Structural basis of histone H2A lysine 119 deubiquitination by Polycomb repressive deubiquitinase BAP1/ASXL1.Science advances · 2023Article
- ALS-linked CCNF variant disrupts motor neuron ubiquitin homeostasis.Human molecular genetics · 2023Article
- Structural basis of histone H2A lysine 119 deubiquitination by Polycomb Repressive Deubiquitinase BAP1/ASXL1.bioRxiv : the preprint server for biology · 2023Article
- Real-Time Deubiquitination Assays Using a Free Ubiquitin Sensor.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Observing Real-Time Ubiquitination in High Throughput with Fluorescence Polarization.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Generation of site-specific ubiquitinated histones through chemical ligation to probe the specificities of histone deubiquitinases.Frontiers in epigenetics and epigenomics · 2023Article
- Probing protein ubiquitination in live cells.Nucleic acids research · 2022Article
- A versatile new tool derived from a bacterial deubiquitylase to detect and purify ubiquitylated substrates and their interacting proteins.PLoS biology · 2022Article
- Role of the Ubiquitin System in Stress Granule Metabolism.International journal of molecular sciences · 2022Review
- Article
- Comparative profiling of stress granule clearance reveals differential contributions of the ubiquitin system.Life science alliance · 2021Article
- Design of genetically encoded sensors to detect nucleosome ubiquitination in live cells.The Journal of cell biology · 2021Article
- Article
- The diversity of linkage-specific polyubiquitin chains and their role in synaptic plasticity and memory formation.Neurobiology of learning and memory · 2020Review
- A deubiquitylase with an unusually high-affinity ubiquitin-binding domain from the scrub typhus pathogen Orientia tsutsugamushi.Nature communications · 2020Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Ubiquitin (Ub) conjugation is an essential post-translational modification that affects nearly all proteins in eukaryotes. The functions and mechanisms of ubiquitination are areas of extensive study, and yet the dynamics and regulation of even free (that is, unconjugated) Ub are poorly understood. A major impediment has been the lack of simple and robust techniques to quantify Ub levels in cells and to monitor Ub release from conjugates. Here, we describe avidity-based fluorescent sensors that address this need. The sensors bind specifically to free Ub, have dissociation constant K
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.