ArticleNature communications2019
Optogenetic control of Bacillus subtilis gene expression.
Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
48 citing papers in PubMed, 103 citations in OpenAlex.
- Genomic analysis highlights a novel class of DPCF-type cyanobacteriochrome.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2026Article
- Single-cell analysis and control of microbial systems using optogenetics.Current opinion in microbiology · 2026Review
- Genetic constraints on protein loop evolution: out-of-frame stop codons limitFrontiers in molecular biosciences · 2026Article
- A stationary phase-specific bacterial green light sensor for enhancing metabolite production.Nature communications · 2025Article
- Phase-driven rewiring in Escherichia coli enhances coenzyme Q10 biosynthesis via temporal and energetic coordination.Applied microbiology and biotechnology · 2025Article
- Multichassis Expression of Cyanobacterial and Other Bacterial Biosynthetic Gene Clusters.ACS synthetic biology · 2025Article
- Changes in Spo0A~P pulsing frequency control biofilm matrix deactivation.PLoS computational biology · 2025Article
- Development and construction of a novel Bacillus subtilis autoinducible extracellular expression system based on a LuxI/R device.Microbial cell factories · 2025Article
- AACS synthetic biology · 2025Article
- Engineered bacteria for near-infrared light-inducible expression of cancer therapeutics.Nature cancer · 2025Article
- Optogenetic Control of B. subtilis Gene Expression Using the CcaSR System.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Light-dependent modulation of protein localization and function in living bacteria cells.Nature communications · 2024Article
- Optogenetic control of Corynebacterium glutamicum gene expression.Nucleic acids research · 2024Article
- Rational Design of Key Enzymes to Efficiently Synthesize Phycocyanobilin inBiomolecules · 2024Article
- Synthetic Homoserine Lactone Sensors for Gram-PositiveACS synthetic biology · 2024Article
- Impacts of UV radiation on Bacillus biocontrol agents and their resistance mechanisms.World journal of microbiology & biotechnology · 2024Review
- Optogenetic control of gene expression in the cyanobacteriumFrontiers in bioengineering and biotechnology · 2024Article
- Engineering Green-light-responsive Heterologous Gene Expression in Pseudomonas.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Accelerating Genetic Sensor Development, Scale-up, and Deployment Using Synthetic Biology.Biodesign research · 2024Article
- Highlighter: An optogenetic system for high-resolution gene expression control in plants.PLoS biology · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
The Gram-positive bacterium Bacillus subtilis exhibits complex spatial and temporal gene expression signals. Although optogenetic tools are ideal for studying such processes, none has been engineered for this organism. Here, we port a cyanobacterial light sensor pathway comprising the green/red photoreversible two-component system CcaSR, two metabolic enzymes for production of the chromophore phycocyanobilin (PCB), and an output promoter to control transcription of a gene of interest into B. subtilis. Following an initial non-functional design, we optimize expression of pathway genes, enhance PCB production via a translational fusion of the biosynthetic enzymes, engineer a strong chimeric output promoter, and increase dynamic range with a miniaturized photosensor kinase. Our final design exhibits over 70-fold activation and rapid response dynamics, making it well-suited to studying a wide range of gene regulatory processes. In addition, the synthetic biology methods we develop to port this pathway should make B. subtilis easier to engineer in the future.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.