Evidence map›Paper›PMID 31306585›Full record

ArticleThe American journal of sports medicine2019

Stem Cell-Conditioned Medium Promotes Graft Remodeling of Midsubstance and Intratunnel Incorporation After Anterior Cruciate Ligament Reconstruction in a Rat Model.

Yaying Sun, Wenbo Chen, Yuefeng Hao, Xueping Gu, Xingwang Liu, Jiangyu Cai, Shaohua Liu, Jiwu Chen, Shiyi Chen

Erratum issuedAbstract readEvaluation Study
PubMed Publisher
In one paragraph

Article in The American journal of sports medicine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 39 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
39citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

39 citing papers in PubMed, 1 synthesis or guideline pooled it.

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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

Yaying SunDepartment of Sports Medicine, Huashan Hospital, Fudan University, Shanghai, China.
Wenbo ChenDepartment of Sports Medicine, Huashan Hospital, Fudan University, Shanghai, China.
Yuefeng HaoDepartment of Orthopaedic Surgery, Northern Branch of Suzhou Municipal Hospital, Suzhou, China.
Xueping GuDepartment of Orthopaedic Surgery, Northern Branch of Suzhou Municipal Hospital, Suzhou, China.
Xingwang LiuDepartment of Sports Medicine, Huashan Hospital, Fudan University, Shanghai, China.
Jiangyu CaiDepartment of Sports Medicine, Huashan Hospital, Fudan University, Shanghai, China.
Shaohua LiuDepartment of Sports Medicine, Huashan Hospital, Fudan University, Shanghai, China.
Jiwu ChenDepartment of Sports Medicine, Huashan Hospital, Fudan University, Shanghai, China.
Shiyi ChenDepartment of Sports Medicine, Huashan Hospital, Fudan University, Shanghai, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundStem cell-conditioned medium (CM) has been increasingly used in regenerative medicine. However, its effect on graft-host integration after anterior cruciate ligament (ACL) reconstruction (ACLR) remains unclear. PURPOSE: To examine the effect of human bone marrow stem cell (hBMSC)-CM on graft-bone integration and graft midsubstance ligamentization in a rat model of ACLR. STUDY

designControlled laboratory study.

methodsCM was obtained from the supernatant of commercially available hBMSCs in serum-free Dulbecco's modified Eagle medium (DMEM). In a rat model of an ACL injury, isometric ACLR was performed. Three groups were established: CM injection group (CM; n = 40), control injection group (CI; n = 40) with serum-free DMEM injections, and no injection group (NI; n = 40). An intra-articular injection was performed weekly. Micro-computed tomography was conducted at 2, 4, and 8 weeks postoperatively. Histological and biomechanical analyses were conducted at 4 and 8 weeks postoperatively. The NIH3T3 fibroblast was utilized as a model in vitro to examine the effect of CM using the cell counting kit-8 (CCK-8) assay and immunofluorescence staining of Ki-67, α-smooth muscle actin (α-SMA), and collagen 1 (Col 1).

resultsAt 4 and 8 weeks, the femoral and tibial bone tunnel areas as well as the interface between the graft and host bone were smaller, while the bone volume/total volume ratio was higher, in the CM group. Sharpey-like fibers formed at 8 weeks in the CM group. At 4 and 8 weeks, more Col 1 was noticed in the CM group than in the NI group (both

conclusionhBMSC-CM accelerates graft-bone incorporation and midsubstance ligamentization and enhances the proliferation, differentiation, and collagen synthesis of fibroblasts. CLINICAL RELEVANCE: Graft-host integration is essential after ACLR. The current study identified a novel agent, that is, hBMSC-CM, as a candidate for promoting integration.

Indexed as

Anterior Cruciate Ligament ReconstructionCulture Media, ConditionedStem CellsAnimalsAnterior Cruciate LigamentAnterior Cruciate Ligament InjuriesBone and BonesFemurHumansMaleMiceNIH 3T3 CellsPostoperative CareRatsTibiaX-Ray MicrotomographyCulture Media, Conditionedanterior cruciate ligament reconstructionconditioned mediumligamentizationtendon-bone healing

Identifiers

PMID31306585

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.