ArticleMikrochimica acta2019
Electrochemical sandwich aptasensor for the carcinoembryonic antigen using graphene quantum dots, gold nanoparticles and nitrogen doped graphene modified electrode and exploiting the peroxidase-mimicking activity of a G-quadruplex DNAzyme.
Article in Mikrochimica acta, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Rapid detection of carcinoembryonic antigen by means of an electrochemical aptasensor.iScience · 2024Article
- Modern Electrochemical Biosensing Based on Nucleic Acids and Carbon Nanomaterials.Sensors (Basel, Switzerland) · 2023Review
- The recent advancements in the early detection of cancer biomarkers by DNAzyme-assisted aptasensors.Journal of nanobiotechnology · 2022Review
- A ratiometric electrochemical DNA-biosensor for detection of miR-141.Mikrochimica acta · 2022Article
- Aptamer-based biosensors and application in tumor theranostics.Cancer science · 2022Review
- Electrochemistry/Photoelectrochemistry-Based Immunosensing and Aptasensing of Carcinoembryonic Antigen.Sensors (Basel, Switzerland) · 2021Review
- Electrochemiluminescence biosensor based on molybdenum disulfide-graphene quantum dots nanocomposites and DNA walker signal amplification for DNA detection.Mikrochimica acta · 2021Article
- Review
- An electrochemical DNA biosensor based on nitrogen-doped graphene nanosheets decorated with gold nanoparticles for genetically modified maize detection.Mikrochimica acta · 2020Article
- Electrochemical immunoassay for the carcinoembryonic antigen based on Au NPs modified zeolitic imidazolate framework and ordered mesoporous carbon.Mikrochimica acta · 2020Article
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3 authors.
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Abstract
A sandwich-type electrochemical aptasensor has been constructed and applied for sensitive and selective detection of the carcinoembryonic antigen (CEA). The surface of a glassy carbon electrode (GCE) was first modified with nitrogen-doped graphene and then gold nanoparticles and graphene quantum dots electrodeposited on it to obtain an architecture of type GQD/AuNP/NG/GCE. In the next step, the CEA-binding aptamer was immobilized on the modified GCE. Hemin intercalates in the amino-modified hemin aptamer to form a hemin-G-quadruplex (hemin-G4) DNAzyme. The amino modified CEA aptamer II is connected to hemin-G4 by glutaraldehyde (GA) as a linker to produce CEAaptamerII/GA/hemin-G4 (=ApII/GA/DNAzyme). Through a sandwich mode, the ApII/GA/DNAzyme bioconjugates are captured on the modified GCE. Subsequently, the hemin-G4 acts as peroxidase-mimicking DNAzyme and rapidly catalyzes the electroreduction of hydrogen peroxide. The quantitative determination of CEA was achieved by differential pulse voltammetry, best at a working potential of around -0.27 V vs. Ag/AgCl. Under optimized conditions, the assay has a linear response in the 10.0 fg mL
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