ArticleNature protocols2019
Creating functional chromosome fusions in yeast with CRISPR-Cas9.
Article in Nature protocols, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed.
- Artificial chromosome reorganization reveals high plasticity of the budding and fission yeast genomes.Genome biology · 2025Article
- GKNnet: an relational graph convolutional network-based method with knowledge-augmented activation layer for microbial structural variation detection.Briefings in bioinformatics · 2025Article
- Elimination of subtelomeric repeat sequences exerts little effect on telomere essential functions ineLife · 2024Article
- Practical Approaches for the YeastInternational journal of molecular sciences · 2023Review
- Sexual Dimorphism through the Lens of Genome Manipulation, Forward Genetics, and Spatiotemporal Sequencing.Genome biology and evolution · 2021Article
- Reconstruction of Secondary Metabolic Pathway to Synthesize Novel Metabolite inFrontiers in bioengineering and biotechnology · 2021Article
- Article
- The CRISPR toolbox in medical mycology: State of the art and perspectives.PLoS pathogens · 2020Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CRISPR-Cas9-facilitated functional chromosome fusion allows the generation of a series of yeast strains with progressively reduced chromosome numbers that are valuable resources for the study of fundamental concepts in chromosome biology, including replication, recombination and segregation. We created a new yeast strain with a single chromosome by using the protocol for chromosome fusion described herein. To ensure the accuracy of chromosome fusions in yeast, the long redundant repetitive sequences near linear chromosomal ends are deleted, and the fusion orders are correspondingly determined. Possible influence on gene expression is minimized to retain gene functionality. This protocol provides experimentally derived guidelines for the generation of functional chromosome fusions in yeast, especially for the deletion of repetitive sequences, the determination of the fusion order and cleavage sites, and primary evaluation of the functionality of chromosome fusions. Beginning with design, one round of typical chromosome fusion and functional verifications can be accomplished within 18 d.
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Registered trials
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