ArticleScientific reports2019
A unified multi-kingdom Golden Gate cloning platform.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- The plant Golden Gate toolkit: compatibility, interoperability, and harmonized assembly in plant synthetic biology.Plant methods · 2026Review
- dSaCas9 enables enhanced transcriptional activation in Nicotiana benthamiana compared to its dSpCas9 ortholog.BMC plant biology · 2026Article
- A red/blue optoswitch for temporal control of chloroplast transcription and biogenesis in Arabidopsis.Nature communications · 2026Article
- A voyage of reprogrammable metabolic bioengineering reshapes plant defense: from editing tools to synthetic systems.Frontiers in plant science · 2026Review
- Guard-cell expression of abscisic acid receptors for engineering water-use-efficient plants without trade-offs in growth.The New phytologist · 2025Article
- Reverse engineering of the pattern recognition receptor FLS2 reveals key design principles of broader recognition spectra against evading flg22 epitopes.Nature plants · 2025Article
- Construction and Characterization of MoClo-Compatible Vectors for Modular Protein Expression inACS synthetic biology · 2025Article
- A Golden Gate compatible system for continuous directed evolution inSynthetic biology (Oxford, England) · 2025Article
- biGMamAct: efficient CRISPR/Cas9-mediated docking of large functional DNA cargoes at theSynthetic biology (Oxford, England) · 2025Article
- Assembling DNA Plasmids with the Multi-Kingdom (MK) Cloning System.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Using ApE for In Silico Golden Gate Cloning.Methods in molecular biology (Clifton, N.J.) · 2025Article
- A peptide-receptor module links cell wall integrity sensing to pattern-triggered immunity.Nature plants · 2024Article
- Allosteric activation of the co-receptor BAK1 by the EFR receptor kinase initiates immune signaling.eLife · 2024Article
- Article
- A divergent protein kinase A regulatory subunit essential for morphogenesis of the human pathogen Leishmania.PLoS pathogens · 2024Article
- Recurrent evolutionary switches of mitochondrial cytochrome c maturation systems in Archaeplastida.Nature communications · 2024Article
- Golden Standard: a complete standard, portable, and interoperative MoClo tool for model and non-model proteobacteria.Nucleic acids research · 2023Article
- Comparison of plastid proteomes points towards a higher plastidial redox turnover in vascular tissues than in mesophyll cells.Journal of experimental botany · 2023Article
- A Modular Cloning Toolkit Including CRISPRi for the Engineering of the Human Fungal Pathogen and Biotechnology HostACS synthetic biology · 2023Article
- Natural and engineered cyclodipeptides: Biosynthesis, chemical diversity, and engineering strategies for diversification and high-yield bioproduction.Engineering microbiology · 2023Review
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Assembling composite DNA modules from custom DNA parts has become routine due to recent technological breakthroughs such as Golden Gate modular cloning. Using Golden Gate, one can efficiently assemble custom transcription units and piece units together to generate higher-order assemblies. Although Golden Gate cloning systems have been developed to assemble DNA plasmids required for experimental work in model species, they are not typically applicable to organisms from other kingdoms. Consequently, a typical molecular biology laboratory working across kingdoms must use multiple cloning strategies to assemble DNA constructs for experimental assays. To simplify the DNA assembly process, we developed a multi-kingdom (MK) Golden Gate assembly platform for experimental work in species from the kingdoms Fungi, Eubacteria, Protista, Plantae, and Animalia. Plasmid backbone and part overhangs are consistent across the platform, saving both time and resources in the laboratory. We demonstrate the functionality of the system by performing a variety of experiments across kingdoms including genome editing, fluorescence microscopy, and protein interaction assays. The versatile MK system therefore streamlines the assembly of modular DNA constructs for biological assays across a range of model organisms.
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