ArticleBMC biotechnology2019
Production of alkaline pectinase: a case study investigating the use of tobacco stalk with the newly isolated strain Bacillus tequilensis CAS-MEI-2-33.
Article in BMC biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 35 citations in OpenAlex.
- Eco-applicable pectinase from Bacillus infantis (SA2): enzyme production, optimization, protein profiling, and pesticide degradation approaches using molecular docking.Archives of microbiology · 2026Article
- Review
- Molecular Characterization of a Novel Alkaline Endo-Pectate Lyase fromMolecules (Basel, Switzerland) · 2025Article
- Isolation and identification of Alcaligenes faecalis W2-3 with high-yield production of dimethyl disulfide.Scientific reports · 2025Article
- Shaping the future of tobacco through microbial insights: a review of advances and applications.Frontiers in bioengineering and biotechnology · 2025Review
- Purification and characterization of the low molecular weight xylanase from Bacillus cereus L-1.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Flow-cytometric cell sorting coupled with UV mutagenesis for improving pectin lyase expression.Frontiers in bioengineering and biotechnology · 2023Article
- Improvement on Thermostability of Pectate Lyase and Its Potential Application to Ramie Degumming.Polymers · 2022Article
- Conversion of Pectin-Containing By-Products to Pectinases byPolymers · 2021Article
- Biochemical Prospects of Various Microbial Pectinase and Pectin: An Approachable Concept in Pharmaceutical Bioprocessing.Frontiers in nutrition · 2020Review
- Genomic, Transcriptomic and Enzymatic Insight into Lignocellulolytic System of a Plant PathogenBiomolecules · 2019Article
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTobacco stalk (TS), a major agricultural waste abundant in pectin, has resulted in concerns about the need for its reuse. The nicotine in TS is considered a chemical that is to\xic and hazardous to the environment.
resultsIn this study, Bacillus tequilensis CAS-MEI-2-33 was isolated from cigar wrappers to produce alkaline pectinase using TS. Subsequently, the medium and fermentation conditions for the production of pectinase by B. tequilensis CAS-MEI-2-33 were optimized. The optimal fermentation period, pH of the initial fermentation medium, concentration of TS, and inoculum amount for B. tequilensis CAS-MEI-2-33 were 40 h, 40 g/L, 7.0, and 3%, respectively. Under optimal conditions, the pectinase activity was 1370 U/mL. Then, the enzymatic properties, such as the optimum pH, reaction temperature, temperature stability, and effects of metal ions, were studied. The optimal pH was determined to be 10.0, indicating that the enzyme was an alkaline pectinase. The optimal temperature was 40 °C, and pectinase activity was stable at 40 °C. The Ag
conclusionsThis study provided a new alkaline pectinase candidate and a new strategy for the use of TS.
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