ArticleNucleic acids research2019
A role for the Saccharomyces cerevisiae ABCF protein New1 in translation termination/recycling.
Article in Nucleic acids research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Article
- Yeast elongation factor homolog New1 protects a subset of mRNAs from degradation by no-go decay.Nucleic acids research · 2026Article
- The ABCF ATPase New1 resolves translation termination defects associated with specific tRNAArg and tRNALys isoacceptors in the P site.Nucleic acids research · 2024Article
- The ABCF ATPase New1 resolves translation termination defects associated with specific tRNAbioRxiv : the preprint server for biology · 2024Article
- Noncanonical usage of stop codons in ciliates expands proteins with structurally flexible Q-rich motifs.eLife · 2024Article
- How Big Is the Yeast Prion Universe?International journal of molecular sciences · 2023Review
- Structural basis for PoxtA-mediated resistance to phenicol and oxazolidinone antibiotics.Nature communications · 2022Article
- A Multi-Perspective Proximity View on the Dynamic Head Region of the Ribosomal 40S Subunit.International journal of molecular sciences · 2021Article
- Context-specific action of macrolide antibiotics on the eukaryotic ribosome.Nature communications · 2021Article
- Yeast translation elongation factor eEF3 promotes late stages of tRNA translocation.The EMBO journal · 2021Article
- ABC-F translation factors: from antibiotic resistance to immune response.FEBS letters · 2021Review
- Ribo-Seq and RNA-Seq ofF1000Research · 2021Article
- Assessing Ribosome Distribution Along Transcripts with Polarity Scores and Regression Slope Estimates.Methods in molecular biology (Clifton, N.J.) · 2021Article
- RiboDiPA: a novel tool for differential pattern analysis in Ribo-seq data.Nucleic acids research · 2020Article
- Comprehensive classification of ABC ATPases and their functional radiation in nucleoprotein dynamics and biological conflict systems.Nucleic acids research · 2020Review
- A unified dinucleotide alphabet describing both RNA and DNA structures.Nucleic acids research · 2020Article
- CTELS: A Cell-Free System for the Analysis of Translation Termination Rate.Biomolecules · 2020Article
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13 authors.
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Abstract
Translation is controlled by numerous accessory proteins and translation factors. In the yeast Saccharomyces cerevisiae, translation elongation requires an essential elongation factor, the ABCF ATPase eEF3. A closely related protein, New1, is encoded by a non-essential gene with cold sensitivity and ribosome assembly defect knock-out phenotypes. Since the exact molecular function of New1 is unknown, it is unclear if the ribosome assembly defect is direct, i.e. New1 is a bona fide assembly factor, or indirect, for instance due to a defect in protein synthesis. To investigate this, we employed yeast genetics, cryo-electron microscopy (cryo-EM) and ribosome profiling (Ribo-Seq) to interrogate the molecular function of New1. Overexpression of New1 rescues the inviability of a yeast strain lacking the otherwise strictly essential translation factor eEF3. The structure of the ATPase-deficient (EQ2) New1 mutant locked on the 80S ribosome reveals that New1 binds analogously to the ribosome as eEF3. Finally, Ribo-Seq analysis revealed that loss of New1 leads to ribosome queuing upstream of 3'-terminal lysine and arginine codons, including those genes encoding proteins of the cytoplasmic translational machinery. Our results suggest that New1 is a translation factor that fine-tunes the efficiency of translation termination or ribosome recycling.
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