ArticleBiology of reproduction2019
Auxin-inducible protein degradation as a novel approach for protein depletion and reverse genetic discoveries in mammalian oocytes†.
Article in Biology of reproduction, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 25 citations in OpenAlex.
- Auxin-Induced Degradation of Target Proteins in Pichia pastoris.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Protein Depletion in Caenorhabditis elegans Using the Auxin-Inducible Degradation System.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Specific auxin and medium combinations alterbioRxiv : the preprint server for biology · 2025Article
- Protein-targeting reverse genetic approaches: the future of oocyte and preimplantation embryo research.Molecular human reproduction · 2025Review
- Specific auxin and medium combinations altermicroPublication biology · 2025Article
- Rapid, efficient auxin-inducible protein degradation inmSphere · 2023Article
- Rapid, efficient auxin-inducible protein degradation inbioRxiv : the preprint server for biology · 2023Article
- Phosphoproteomic Approaches for Identifying Phosphatase and Kinase Substrates.Molecules (Basel, Switzerland) · 2023Review
- Polo-like kinase 1 promotes Cdc42-induced actin polymerization for asymmetric division in oocytes.Open biology · 2023Article
- Rapid COG Depletion in Mammalian Cell by Auxin-Inducible Degradation System.Methods in molecular biology (Clifton, N.J.) · 2023Article
- A spontaneousmicroPublication biology · 2023Article
- Small molecule-inducible gene regulatory systems in mammalian cells: progress and design principles.Current opinion in biotechnology · 2022Review
- Applying the auxin-based degron system for the inducible, reversible and complete protein degradation iniScience · 2022Article
- Article
- Auxin confers protection against ER stress inBiology open · 2021Article
- Efficient generation of a single-copymicroPublication biology · 2021Article
- Adaptation of the AID system for stem cell and transgenic mouse research.Stem cell research · 2020Article
- Review
- Auxin-mediated Protein Degradation inBio-protocol · 2020Article
- Rapid Degradation ofG3 (Bethesda, Md.) · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The disruption of protein expression is a major approach used for investigating protein function in mammalian oocytes. This is often achieved with RNAi/morpholino-mediated knockdown or gene knockout, leading to long-term loss of proteins of interest. However, these methods have noteworthy limitations, including (a) slow protein turnover can prohibit use of these approaches; (b) essential roles in early events precludes characterization of functions in subsequent events; (c) extended protein loss can allow time for compensatory mechanisms and other unanticipated events that confound interpretation of results. The work presented here examines the use of auxin-inducible degradation, a powerful new approach that overcomes these limitations through the depletion of one's protein of interest through controllable ubiquitin-mediated degradation. This method has been employed in yeast and mammalian cell lines, and here we demonstrate the utility of auxin-inducible degradation in mouse oocytes at multiple stages of meiosis, through degradation of exogenously expressed EGFP. We also evaluate important parameters for experimental design for use of this system in oocytes. This study thus expands the toolkit of researchers in oocyte biology, establishing the use of this unique and versatile approach for depleting proteins in oocytes, and providing researchers with valuable information to make use of this system.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.