ArticleEnvironmental microbiology2019
Highly variable mRNA half-life time within marine bacterial taxa and functional genes.
Article in Environmental microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed.
- Phytoplankton growth and potential cyanotoxin production differ in response to nitrogen and phosphorus amendments in late summer communities from Kabetogama Lake (Minnesota, United States).Journal of phycology · 2026Article
- Article
- Article
- Depth-dependent distribution patterns of ammonia- and nitrite-oxidizing microorganisms in the water column of stratified lakes.Scientific reports · 2025Article
- Spatiotemporal and temperature-dependent disconnect between ammonia oxidation and dark DIC fixation in deep oligotrophic Lake Constance.ISME communications · 2025Article
- Small regulatory RNA RSaX28 promotes virulence by reinforcing the stability of RNAIII in community-associated ST398 clonotypeEmerging microbes & infections · 2024Article
- Substrate uptake patterns shape niche separation in marine prokaryotic microbiome.Science advances · 2024Article
- Widespread and active piezotolerant microorganisms mediate phenolic compound degradation under high hydrostatic pressure in hadal trenches.Marine life science & technology · 2024Article
- Seasonal patterns in microbial carbon and iron transporter expression in the Southern Ocean.Microbiome · 2023Article
- A Fast and Easy Method to Co-extract DNA and RNA from an Environmental Microbial Sample.Microbes and environments · 2023Article
- Complex marine microbial communities partition metabolism of scarce resources over the diel cycle.Nature ecology & evolution · 2022Article
- One Cell at a Time: Advances in Single-Cell Methods and Instrumentation for Discovery in Aquatic Microbiology.Frontiers in microbiology · 2022Article
- A small regulatory RNA alters Staphylococcus aureus virulence by titrating RNAIII activity.Nucleic acids research · 2021Article
- Metatranscriptomics bymSystems · 2020Article
- Dormancy dampens the microbial distance-decay relationship.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
Messenger RNA can provide valuable insights into the variability of metabolic processes of microorganisms. However, due to uncertainties that include the stability of RNA, its application for activity profiling of environmental samples is questionable. We explored different factors affecting the decay rate of transcripts of three marine bacterial isolates using qPCR and determined mRNA half-life time of specific bacterial taxa and of functional genes by metatranscriptomics of a coastal environmental prokaryotic community. The half-life time of transcripts from 11 genes from bacterial isolates ranged from 1 to 46 min. About 80% of the analysed transcripts exhibited half-live times shorter than 10 min. Significant differences were found in the half-life time between mRNA and rRNA. The half-life time of mRNA obtained from a coastal metatranscriptome ranged from 9 to 400 min. The shortest half-life times of the metatranscriptome corresponded to transcripts from the same clusters of orthologous groups (COGs) in all bacterial classes. The prevalence of short mRNA half-life time in genes related to defence mechanisms and motility indicate a tight connection of RNA decay rate to environmental stressors. The short half-life time of RNA and its high variability needs to be considered when assessing metatranscriptomes especially in environmental samples.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.