Evidence map›Paper›PMID 31298767›Full record

ArticleStem cells (Dayton, Ohio)2020

Tunneling nanotubes mediate the expression of senescence markers in mesenchymal stem/stromal cell spheroids.

Jacklyn Whitehead, Jiali Zhang, Jenna N Harvestine, Alefia Kothambawala, Gang-Yu Liu, J Kent Leach

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells (Dayton, Ohio), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed
4.7field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed, 53 citations in OpenAlex.

  1. Review
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  7. Tunneling Nanotubes in the Brain.Results and problems in cell differentiation · 2024
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Jacklyn WhiteheadDepartment of Biomedical Engineering, University of California, Davis, California.
Jiali ZhangDepartment of Chemistry, University of California, Davis, California.
Jenna N HarvestineDepartment of Biomedical Engineering, University of California, Davis, California.
Alefia KothambawalaDepartment of Biomedical Engineering, University of California, Davis, California.
Gang-Yu LiuDepartment of Chemistry, University of California, Davis, California.
J Kent LeachDepartment of Biomedical Engineering, University of California, Davis, California.
University of California, Davis · USUC Davis Health · US

Funding

Staff InvestigatorsP30CA093373 · NCI · UNIVERSITY OF CALIFORNIA DAVIS · PI Laura Marcu · 2002 to 2026
$84.9M
Biomaterial regulation of cell spheroids to synergistically enhance bone healingR01DE025475 · NIDCR · UNIVERSITY OF CALIFORNIA AT DAVIS · PI LEACH, J. KENT · 2015 to 2019
$1.9M
A New Approach for Probing Interactions between Nanomaterials and CellsR21ES025350 · NIEHS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI LIU, GANG-YU · 2015 to 2016
$417k
NIDCR NIH HHS R01 DE025475NIEHS NIH HHS R21 ES025350
6 · The paper itself

Abstract

The therapeutic potential of mesenchymal stem/stromal cells (MSCs) is limited by acquired senescence following prolonged culture expansion and high-passage numbers. However, the degree of cell senescence is dynamic, and cell-cell communication is critical to promote cell survival. MSC spheroids exhibit improved viability compared with monodispersed cells, and actin-rich tunneling nanotubes (TNTs) may mediate cell survival and other functions through the exchange of cytoplasmic components. Building upon our previous demonstration of TNTs bridging MSCs within these cell aggregates, we hypothesized that TNTs would influence the expression of senescence markers in MSC spheroids. We confirmed the existence of functional TNTs in MSC spheroids formed from low-passage, high-passage, and mixtures of low- and high-passage cells using scanning electron microscopy, confocal microscopy, and flow cytometry. The contribution of TNTs toward the expression of senescence markers was investigated by blocking TNT formation with cytochalasin D (CytoD), an inhibitor of actin polymerization. CytoD-treated spheroids exhibited decreases in cytosol transfer. Compared with spheroids formed solely of high-passage MSCs, the addition of low-passage MSCs reduced p16 expression, a known genetic marker of senescence. We observed a significant increase in p16 expression in high-passage cells when TNT formation was inhibited, establishing the importance of TNTs in MSC spheroids. These data confirm the restorative role of TNTs within MSC spheroids formed with low- and high-passage cells and represent an exciting approach to use higher-passage cells in cell-based therapies.

Indexed as

BiomarkersCellular SenescenceHumansMesenchymal Stem CellsNanotubesBiomarkersmesenchymal stem/stromal cellsp16senescencespheroidstunneling nanotubes

Identifiers

PMID31298767
PMCPMC6954984
OpenAlexW2959409583

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.