ArticleCell proliferation2019
Enhanced bone tissue regeneration of a biomimetic cellular scaffold with co-cultured MSCs-derived osteogenic and angiogenic cells.
Article in Cell proliferation, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 36 citations in OpenAlex.
- Injectable Hydroxyapatite and Tetracalcium Phosphate Granules Combined with Autologous Bone Marrow Concentrate: Toward Shape-Adaptive, Single-Step Bone Regeneration.Journal of functional biomaterials · 2026Article
- Mussel-inspired silica, strontium, and fluoride-doped hydroxyapatite/BMP-2 composite coating: a new strategy to enhance the bioactivity of zirconia implants.RSC advances · 2026Article
- Nanozymes for Bone Regeneration: Mechanistic Insights into Immune and Metabolic Microenvironment Modulation.International journal of nanomedicine · 2026Review
- Neuro-immune-vascular-stem cell crosstalk in bone/cartilage regeneration: mechanisms, technological advances, and clinical perspectives.Frontiers in bioengineering and biotechnology · 2026Review
- Enhancing bone regeneration using kaempferol as an osteoprotective compound: signaling mechanisms, delivery strategies, and potential applications.Journal of biological engineering · 2025Review
- Strategies for promoting neurovascularization in bone regeneration.Military Medical Research · 2025Review
- Analysis of the Effect of Human Type I Collagen-Derived Peptide on Bone Regenerative Capacity and Comparison with Various Collagen Materials In Vivo.Medicina (Kaunas, Lithuania) · 2025Article
- Combining "waste utilization" and "tissue to tissue" strategies to accelerate vascularization for bone repair.Journal of orthopaedic translation · 2024Article
- Towards Stem Cell Therapy for Critical-Sized Segmental Bone Defects: Current Trends and Challenges on the Path to Clinical Translation.Journal of functional biomaterials · 2024Review
- Revolutionizing bone defect healing: the power of mesenchymal stem cells as seeds.Frontiers in bioengineering and biotechnology · 2024Review
- Sequential gastrodin release PU/n-HA composite scaffolds reprogram macrophages for improved osteogenesis and angiogenesis.Bioactive materials · 2023Article
- An injectable pH neutral bioactive glass-based bone cement with suitable bone regeneration ability.Journal of orthopaedic translation · 2022Article
- Educating EVs to Improve Bone Regeneration: Getting Closer to the Clinic.International journal of molecular sciences · 2022Review
- Bone Regeneration and Oxidative Stress: An Updated Overview.Antioxidants (Basel, Switzerland) · 2022Review
- Gastrodin modified polyurethane conduit promotes nerve repair via optimizing Schwann cells function.Bioactive materials · 2022Article
- Biological Effects, Applications and Design Strategies of Medical Polyurethanes Modified by Nanomaterials.International journal of nanomedicine · 2022Review
- Additive Manufacturing: The Next Generation of Scapholunate Ligament Reconstruction.Journal of wrist surgery · 2021Article
- MicroRNAs Modulate Signaling Pathways in Osteogenic Differentiation of Mesenchymal Stem Cells.International journal of molecular sciences · 2021Review
- miR-129-5p Promotes Osteogenic Differentiation of BMSCs and Bone Regeneration via Repressing Dkk3.Stem cells international · 2021Article
- In vivo bone regeneration assessment of offset and gradient melt electrowritten (MEW) PCL scaffolds.Biomaterials research · 2020Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
objectivesThe bone tissue engineering primarily focuses on three-dimensional co-culture systems, which physical and biological properties resemble the cell matrix of actual tissues. The complex dialogue between bone-forming and endothelial cells (ECs) in a tissue-engineered construct will directly regulate angiogenesis and bone regeneration. The purpose of this study was to investigate whether co-culture between osteogenic and angiogenic cells derived by bone mesenchymal stem cells (MSCs) could affect cell activities and new bone formation. MATERIALS AND
methodsMesenchymal stem cells were dually induced to differentiate into osteogenic cells (OMSCs) and ECs; both cell types were co-cultured at different ratios to investigate their effects and underlying mechanisms through ELISA, RT-qPCR and MTT assays. The selected cell mixture was transplanted onto a nano-hydroxyapatite/polyurethane (n-HA/PU) scaffold to form a cell-scaffold construct that was implanted in the rat femoral condyles. Histology and micro-CT were examined for further verification.
resultsELISA and gene expression studies revealed that co-cultured OMSCs/ECs (0.5/1.5) significantly elevated the transcription levels of osteogenic genes such as ALP, Col-I and OCN, as well as transcription factors Msx2, Runx2 and Osterix; it also upregulated angiogenic factors of vascular endothelial growth factor (VEGF) and CD31 when compared with cells cultured alone or in other ratios. The optimized OMSCs/ECs group had more abundant calcium phosphate crystal deposition, further facilitated their bone formation in vivo.
conclusionsThe OMSCs/ECs-scaffold constructs at an optimal cell ratio (0.5/1.5) achieved enhanced osteogenic and angiogenic factor expression and biomineralization, which resulted in more effective bone formation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.