ArticleJournal of industrial microbiology & biotechnology2019
Combinatorial expression of different β-carotene hydroxylases and ketolases in Escherichia coli for increased astaxanthin production.
Article in Journal of industrial microbiology & biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Development of an efficient genome editing system using recombinase-mediated cassette exchange inSynthetic and systems biotechnology · 2026Article
- Installing a Ketocarotenoid Branch inMarine drugs · 2025Article
- Effect of Heterologous Expression of Key Enzymes Involved in Astaxanthin and Lipid Synthesis on Lipid and Carotenoid Production inMarine drugs · 2025Article
- Fast and High-Efficiency Synthesis of Capsanthin in Pepper by Transient Expression of Geminivirus.International journal of molecular sciences · 2023Article
- Production of Astaxanthin Using CBFD1/HFBD1 fromBioengineering (Basel, Switzerland) · 2023Article
- Directed evolution of the fusion enzyme for improving astaxanthin biosynthesis inSynthetic and systems biotechnology · 2023Article
- Biotechnological advances for improving natural pigment production: a state-of-the-art review.Bioresources and bioprocessing · 2022Review
- Discovery of Geranylgeranyl Pyrophosphate Synthase (GGPPS) Paralogs fromMarine drugs · 2019Article
- Improved Astaxanthin Production withMarine drugs · 2019Article
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Authors and funding
7 authors.
Funding
Abstract
In natural produced bacteria, β-carotene hydroxylase (CrtZ) and β-carotene ketolase (CrtW) convert β-carotene into astaxanthin. To increase astaxanthin production in heterologous strain, simple and effective strategies based on the co-expression of CrtZ and CrtW were applied in E. coli. First, nine artificial operons containing crtZ and crtW genes from different sources were constructed and, respectively, introduced into E. coli ZF237T, a β-carotene producing host. Among the nine resulting strains, five accumulated detectable amounts of astaxanthin ranging from 0.49 to 8.07 mg/L. Subsequently, the protein fusion CrtZ to CrtW using optimized peptide linkers further increased the astaxanthin production. Strains expressing fusion proteins with CrtZ rather than CrtW attached to the N-terminus accumulated much more astaxanthin. The astaxanthin production of the best strain ZF237T/CrtZ
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