ArticleProtein engineering, design & selection : PEDS2019
User-defined single pot mutagenesis using unamplified oligo pools.
Article in Protein engineering, design & selection : PEDS, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed.
- Article
- An integrated technology for quantitative wide mutational scanning of human antibody Fab libraries.Nature communications · 2024Article
- An integrated technology for quantitative wide mutational scanning of human antibody Fab libraries.bioRxiv : the preprint server for biology · 2024Article
- Standardizing cassette-based deep mutagenesis by Golden Gate assembly.Biotechnology and bioengineering · 2024Article
- Antibody-directed evolution reveals a mechanism for enhanced neutralization at the HIV-1 fusion peptide site.Nature communications · 2023Article
- SUNi mutagenesis: Scalable and uniform nicking for efficient generation of variant libraries.PloS one · 2023Article
- Rapid biosensor development using plant hormone receptors as reprogrammable scaffolds.Nature biotechnology · 2022Article
- Highly protective antimalarial antibodies via precision library generation and yeast display screening.The Journal of experimental medicine · 2022Article
- Oligo Pools as an Affordable Source of Synthetic DNA for Cost-Effective Library Construction in Protein- and Metabolic Pathway Engineering.Chembiochem : a European journal of chemical biology · 2022Review
- Apoptolidin family glycomacrolides target leukemia through inhibition of ATP synthase.Nature chemical biology · 2022Article
- High-Throughput DNA FISH (hiFISH).Methods in molecular biology (Clifton, N.J.) · 2022Article
- One-shot identification of SARS-CoV-2 S RBD escape mutants using yeast screening.Cell reports · 2021Article
- A Method for User-defined Mutagenesis by Integrating Oligo Pool Synthesis Technology with Nicking Mutagenesis.Bio-protocol · 2020Article
- Saturation Mutagenesis Genome Engineering of Infective ΦX174 BacteriophageACS synthetic biology · 2020Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
User-defined mutagenic libraries are fundamental for applied protein engineering workflows. Here we show that unamplified oligo pools can be used to prepare site saturation mutagenesis libraries from plasmid DNA with near-complete coverage of desired mutations and few off-target mutations. We find that oligo pools yield higher quality libraries when compared to individually synthesized degenerate oligos. We also show that multiple libraries can be multiplexed into a single oligo pool, making preparation of multiple libraries less expensive and more convenient. We provide software for automatic oligo pool design that can generate mutagenic oligos for saturating or focused libraries.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.