ArticleBiotechnology for biofuels2019
FT-NIR: a tool for rapid intracellular lipid quantification in oleaginous yeasts.
Article in Biotechnology for biofuels, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 28 citations in OpenAlex.
- Review
- Enhanced glycerol assimilation and lipid production in Rhodotorula toruloides CBS14 upon addition of hemicellulose primarily correlates with early transcription of energy-metabolism-related genes.Biotechnology for biofuels and bioproducts · 2023Article
- Near Chromosome-Level Genome Assembly and Annotation ofJournal of fungi (Basel, Switzerland) · 2022Article
- Rhodotorula toruloides: an ideal microbial cell factory to produce oleochemicals, carotenoids, and other products.World journal of microbiology & biotechnology · 2021Review
- Article
- Oleaginous yeasts respond differently to carbon sources present in lignocellulose hydrolysate.Biotechnology for biofuels · 2021Article
- Microbial lipid production from crude glycerol and hemicellulosic hydrolysate with oleaginous yeasts.Biotechnology for biofuels · 2021Article
- Conversion of sugar beet residues into lipids by Lipomyces starkeyi for biodiesel production.Microbial cell factories · 2020Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundLipid extraction for quantification of fat content in oleaginous yeasts often requires strong acids and harmful organic solvents; it is laborious and time-consuming. Therefore, in most cases just endpoint measurements of lipid accumulation are performed and kinetics of intracellular lipid accumulation is difficult to follow. To address this, we created a prediction model using Fourier-transform near-infrared (FT-NIR) spectroscopy. This method allows to measure lipid content in yeast.
methodsThe FT-NIR calibration sets were constructed from spectra of freeze-dried cells of the oleaginous yeasts
resultsThe
conclusionsUsing FT-NIR spectroscopy, we have developed a faster, less laborious and non-destructive quantification of yeast intracellular lipid content compared to methods using lipid extraction.
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Registered trials
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