ArticleBiotechnology for biofuels2019
Directed evolution of a genetically encoded immobilized lipase for the efficient production of biodiesel from waste cooking oil.
Article in Biotechnology for biofuels, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 58 citations in OpenAlex.
- Enhancing the Versatility of Polyethylene Terephthalate (PET) Through Strategic Biomolecular Functionalization.Angewandte Chemie (International ed. in English) · 2026Review
- An engineered yeast cytosine deaminase with improved catalytic activity and stability for macrophage-mediated enzyme/prodrug therapy.Communications biology · 2025Article
- A Simple Strategy for Co-Immobilization of NADH-Dependent Enzymes and NADH for Efficient Biocatalysis in Continuous Flow Reactors.ChemSusChem · 2025Article
- Magnetizing Biotech-Advances in (In Vivo) Magnetic Enzyme Immobilization.Engineering in life sciences · 2025Review
- Optimisation strategies for directed evolution without sequencing.PLoS computational biology · 2024Article
- Mechanoresponsive Protein Crystals for NADH Recycling in Multicycle Enzyme Reactions.Journal of the American Chemical Society · 2024Article
- A Bioresponsive Genetically Encoded Antimicrobial Crystal for the Oral Treatment of Helicobacter Pylori Infection.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Bioengineering to Accelerate Biodiesel Production for a Sustainable Biorefinery.Bioengineering (Basel, Switzerland) · 2022Review
- Can (We Make)Toxins · 2021Review
- Targeted Myoglobin Delivery as a Strategy for Enhancing the Sensitivity of Hypoxic Cancer Cells to Radiation.iScience · 2020Article
- Directed Evolution ofFrontiers in bioengineering and biotechnology · 2020Article
- Biodiesel and flavor compound production using a novel promiscuous cold-adapted SGNH-type lipase (Biotechnology for biofuels · 2020Article
- Chemical treatments for modification and immobilization to improve the solvent-stability of lipase.World journal of microbiology & biotechnology · 2019Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundWe have recently developed a one-step, genetically encoded immobilization approach based on fusion of a target enzyme to the self-crystallizing protein Cry3Aa, followed by direct production and isolation of the fusion crystals from
resultsGenetic fusion of the
conclusionsThe genetically encoded nature of our Cry3Aa-fusion immobilization platform makes it possible to perform both directed evolution and screening of immobilized enzymes directly in vivo. This work is the first example of the use of directed evolution to optimize an enzyme in its immobilized state allowing for identification of a mutant that would unlikely have been identified from screening of its soluble form. We demonstrate that the resulting Cry3Aa-PML catalyst is suitable for the recyclable conversion of waste cooking oil into biodiesel.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.