ArticleBMC genomics2019
Independent modulation of individual genomic component transcription and a cis-acting element related to high transcriptional activity in a multipartite DNA virus.
Article in BMC genomics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- BBTV Nuclear Shuttle Protein Mediates Banana Ubiquitination Pathway Dysregulation.Plants (Basel, Switzerland) · 2026Article
- Structural Modeling of Protein-DNA Interactions Underlying Genome Copy Number Variation in Nanoviruses.Computational and structural biotechnology journal · 2026Article
- Living Together Apart: Quantitative Perspectives on the Costs and Benefits of a Multipartite Genome Organization in Viruses.Viruses · 2025Article
- Relative frequency dynamics and loading of beet necrotic yellow vein virus genomic RNAs during the acquisition by its vectorJournal of virology · 2025Article
- Aspects of the lifestyle of multipartite viruses apply to monopartite segmented and perhaps nonsegmented viruses.Npj viruses · 2024Review
- Reassortments in single-stranded DNA multipartite viruses: Confronting expectations based on molecular constraints with field observations.Virus evolution · 2024Review
- A new genus of alphasatellites associated with banana bunchy top virus in Southeast Asia.Virus evolution · 2024Article
- The genome formula of a multipartite virus is regulated both at the individual segment and the segment group levels.PLoS pathogens · 2024Article
- Evaluation of sequencing and PCR-based methods for the quantification of the viral genome formula.Virus research · 2023Article
- A newly emerging alphasatellite affects banana bunchy top virus replication, transcription, siRNA production and transmission by aphids.PLoS pathogens · 2022Article
- Gene copy number variations at the within-host population level modulate gene expression in a multipartite virus.Virus evolution · 2022Article
- A New Method to Obtain the Complete Genome Sequence of Multiple-Component Circular ssDNA Viruses by Transcriptome Analysis.Frontiers in bioengineering and biotechnology · 2020Article
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Authors and funding
6 authors.
Funding
Abstract
backgroundThe genome of Banana bunchy top virus (BBTV) consists of at least six circular, single-stranded DNA components of ~ 1 kb in length. Some BBTV isolates may also carry satellite DNA molecules that are not essential for BBTV infection. The relation between multipartite DNA virus replication and their transcriptional levels and the underlying mechanism remain unclear.
resultsTo understand the coordinated replication and transcription of the multiple genomic components, the absolute amounts of each BBTV DNA component were measured by real-time PCR (qPCR), and their transcriptional levels were determined by RNAseq and reverse transcription-qPCR (qRT-PCR). Significant differences were found in the absolute amounts of individual BBTV genomic components. Transcriptional levels of each BBTV genomic component obtained from the RNAseq data matched closely to those obtained from qRT-PCR, but did not correspond to the absolute amount of each DNA component. The ratio of transcript over DNA copies ranged from 46.21 to 1059.44%, which was possibly regulated by the promoter region in the intergenic region of each component. To further determine this speculation, the promoter region of the DNA-S, -M or -N was constructed to the upstream of green fluorescent protein (GFP) gene for transient expression by agrobacterium-mediated transformation method. The qRT-PCR showed the highest transcriptional activity was promoted by DNA-N promoter, about 386.58% activity comparing with CaMV 35S promoter. Confocal microscopy observation showed that the intensity of green fluorescence was corresponding to that of qRT-PCR.
conclusionsOur data clearly showed that BBTV was able to control the transcriptional level of each DNA component independently by through the promoter sequences in the intergenic region. Moreover, a cis-acting element from DNA-N component had a high transcriptional activity.
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