ArticleMatrix biology : journal of the International Society for Matrix Biology2020
Peptide gels of fully-defined composition and mechanics for probing cell-cell and cell-matrix interactions in vitro.
Article in Matrix biology : journal of the International Society for Matrix Biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 59 citations in OpenAlex.
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- Engineering Biomimetic 3D Microenvironments for Extracellular Vesicle Programming Toward Clinical Translation.International journal of molecular sciences · 2026Review
- Hybrid Scaffolds Decouple Biochemical & Biophysical Regulation of Cell Phenotype.Advanced healthcare materials · 2026Article
- Self-Assembly of Off-Target Peptide Sequences: Implications for the Design of Soft Materials.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Virtual testing methodology to predict the mechanical behavior of collagen hydrogels from nanoarchitecture.Materials today. Bio · 2025Article
- Insights into the mechanisms, regulation, and therapeutic implications of extracellular matrix stiffness in cancer.Bioengineering & translational medicine · 2025Review
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- The importance of 3D fibre architecture in cancer and implications for biomaterial model design.Nature reviews. Cancer · 2024Review
- Fully angularly resolved 3D microrheology with optical tweezers.Rheologica acta · 2024Article
- Elastin-like Recombinamer Hydrogels as Platforms for Breast Cancer Modeling.Biomacromolecules · 2023Article
- Selection and Optimization of a Bioink Based on PANC-1- Plasma/Alginate/Methylcellulose for Pancreatic Tumour Modelling.Polymers · 2023Article
- OptoRheo: Simultaneous in situ micro-mechanical sensing and imaging of live 3D biological systems.Communications biology · 2023Article
- Structuring supramolecular hyaluronan hydrogels via peptide self-assembly for modulating the cell microenvironment.Materials today. Bio · 2023Article
- Click chemistry functionalization of self-assembling peptide hydrogels.Journal of biomedical materials research. Part A · 2023Article
- Hydrogel-Based Pre-Clinical Evaluation of Repurposed FDA-Approved Drugs for AML.International journal of molecular sciences · 2023Article
- Development of anF1000Research · 2023Article
- Article
- Application of a 3D hydrogel-based model to replace use of animals for passaging patient-derived xenografts.In vitro models · 2023Article
- Measuring the linear viscoelastic regime of MCF-7 cells with a monolayer rheometer in the presence of microtubule-active anti-cancer drugs at high concentrations.Interface focus · 2022Article
- Review
Corrections and comments
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Authors and funding
14 authors at 3 institutions in 1 country.
Funding
Abstract
Current materials used for in vitro 3D cell culture are often limited by their poor similarity to human tissue, batch-to-batch variability and complexity of composition and manufacture. Here, we present a "blank slate" culture environment based on a self-assembling peptide gel free from matrix motifs. The gel can be customised by incorporating matrix components selected to match the target tissue, with independent control of mechanical properties. Therefore the matrix components are restricted to those specifically added, or those synthesised by encapsulated cells. The flexible 3D culture platform provides full control over biochemical and physical properties, allowing the impact of biochemical composition and tissue mechanics to be separately evaluated in vitro. Here, we demonstrate that the peptide gels support the growth of a range of cells including human induced pluripotent stem cells and human cancer cell lines. Furthermore, we present proof-of-concept that the peptide gels can be used to build disease-relevant models. Controlling the peptide gelator concentration allows peptide gel stiffness to be matched to normal breast (<1 kPa) or breast tumour tissue (>1 kPa), with higher stiffness favouring the viability of breast cancer cells over normal breast cells. In parallel, the peptide gels may be modified with matrix components relevant to human breast, such as collagen I and hyaluronan. The choice and concentration of these additions affect the size, shape and organisation of breast epithelial cell structures formed in co-culture with fibroblasts. This system therefore provides a means of unravelling the individual influences of matrix, mechanical properties and cell-cell interactions in cancer and other diseases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.