ArticlePLoS genetics2019
Reproduction disrupts stem cell homeostasis in testes of aged male Drosophila via an induced microenvironment.
Article in PLoS genetics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 23 citations in OpenAlex.
- Signaling pathways in Drosophila testis niche: Local signals that regulate stem cell fate.Insect science · 2026Review
- Oral intake of aripiprazole compromises male fertility in Drosophila.Biology direct · 2025Article
- Interplay of EGFR, JNK, and ROS signaling in soma-germline communication in the Drosophila testis.Stem cell reports · 2025Article
- A comparison of spermatogenesis between flies and men-conserved processes of male gamete production.Human reproduction update · 2025Review
- Signals from the niche promote distinct modes of translation initiation to control stem cell differentiation and renewal in the Drosophila testis.PLoS biology · 2025Article
- Mating-induced Ecdysone in the testis disrupts soma-germline contacts and stem cell cytokinesis.Development (Cambridge, England) · 2024Article
- Single-cell transcriptome characteristics of testicular terminal epithelium lineages during aging in the Drosophila.Aging cell · 2024Article
- c-Jun N-terminal kinase signaling in aging.Frontiers in aging neuroscience · 2024Review
- Mating-induced ecdysone in the testis disrupts soma-germline contacts and stem cell cytokinesis.bioRxiv : the preprint server for biology · 2023Article
- Single-cell RNA sequencing reveals cell landscape following antimony exposure during spermatogenesis in Drosophila testes.Cell death discovery · 2023Article
- Targeting the stem cell niche micro-environment as therapeutic strategies in aging.Frontiers in cell and developmental biology · 2023Review
- Article
- The Emerging Roles of JNK Signaling inInternational journal of molecular sciences · 2021Review
- Upregulated TNF/Eiger signaling mediates stem cell recovery and tissue homeostasis during nutrient resupply in Drosophila testis.Scientific reports · 2020Article
- CG8005 Mediates Transit-Amplifying Spermatogonial Divisions via Oxidative Stress inOxidative medicine and cellular longevity · 2020Article
- Irreversible effects of youthful choices in aged adults.PLoS genetics · 2019Article
Corrections and comments
- Commented on by
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stem cells rely on instructive cues from their environment. Alterations in microenvironments might contribute to tissue dysfunction and disease pathogenesis. Germline stem cells (GSCs) and cyst stem cells (CySC) in Drosophila testes are normally maintained in the apical area by the testicular hub. In this study, we found that reproduction leads to accumulation of early differentiating daughters of CySCs and GSCs in the testes of aged male flies, due to hyperactivation of Jun-N-terminal kinase (JNK) signaling to maintain self-renewal gene expression in the differentiating cyst cells. JNK activity is normally required to maintain CySCs in the apical niche. A muscle sheath surrounds the Drosophila testis to maintain its long coiled structure. Importantly, reproduction triggers accumulation of the tumor necrosis factor (TNF) Eiger in the testis muscle to activate JNK signaling via the TNF receptor Grindelwald in the cyst cells. Reducing Eiger activity in the testis muscle sheath suppressed reproduction-induced differentiation defects, but had little effect on testis homeostasis of unmated males. Our results reveal that reproduction in males provokes a dramatic shift in the testicular microenvironment, which impairs tissue homeostasis and spermatogenesis in the testes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.