ArticleRadiation research2019
RABiT-II-DCA: A Fully-automated Dicentric Chromosome Assay in Multiwell Plates.
Article in Radiation research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Multicentered Biospecimen Analyses after 4 Gy Sublethal Total Body Irradiation in Rhesus Macaques.Radiation research · 2025Article
- Centers for Medical Countermeasures against Radiation Consortium: Past, Present, and Beyond.Radiation research · 2025Review
- Adaptive Segmentation of DAPI-stained, C-banded, Aggregated and Overlapping Chromosomes.Cell biochemistry and biophysics · 2024Article
- Multiwell-based G0-PCC assay for radiation biodosimetry.Scientific reports · 2024Article
- RABiT-III: an Automated Micronucleus Assay at a Non-Specialized Biodosimetry Facility.Radiation research · 2024Article
- Sex differences in radiation research.International journal of radiation biology · 2024Article
- Sex and dose rate effects in automated cytogenetics.Radiation protection dosimetry · 2023Article
- RENEB Inter-Laboratory Comparison 2021: Inter-Assay Comparison of Eight Dosimetry Assays.Radiation research · 2023Article
- High Resolution and Automatable Cytogenetic Biodosimetry Using In Situ Telomere and Centromere Hybridization for the Accurate Detection of DNA Damage: An Overview.International journal of molecular sciences · 2023Review
- High-precision automatic identification method for dicentric chromosome images using two-stage convolutional neural network.Scientific reports · 2023Article
- Validation of a High-Throughput Dicentric Chromosome Assay Using Complex Radiation Exposures.Radiation research · 2023Article
- Transcriptomics for radiation biodosimetry: progress and challenges.International journal of radiation biology · 2023Review
- A machine learning method for improving the accuracy of radiation biodosimetry by combining data from the dicentric chromosomes and micronucleus assays.Scientific reports · 2022Article
- Nuclear and Radiological Emergencies: Biological Effects, Countermeasures and Biodosimetry.Antioxidants (Basel, Switzerland) · 2022Review
- Whole blood gene expression within days after total-body irradiation predicts long term survival in Gottingen minipigs.Scientific reports · 2021Article
- Transportation container for pre-processing cytogenetic assays in radiation accidents.Scientific reports · 2021Article
- Machine learning methodology for high throughput personalized neutron dose reconstruction in mixed neutron + photon exposures.Scientific reports · 2021Article
- The RABiT-II DCA in the Rhesus Macaque Model.Radiation research · 2020Article
- Dose and Dose-Rate Effects in a Mouse Model of Internal Exposure from 137Cs. Part 2: Integration of Gamma-H2AX and Gene Expression Biomarkers for Retrospective Radiation Biodosimetry.Radiation research · 2020Article
- Gene Expression Profiles from Heart, Lung and Liver Samples of Total-Body-Irradiated Minipigs: Implications for Predicting Radiation-Induced Tissue Toxicity.Radiation research · 2020Article
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Authors and funding
6 authors.
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Abstract
We developed a fully-automated dicentric chromosome assay (DCA) in multiwell plates. All operations, from sample loading to chromosome scoring, are performed, without human intervention, by the second-generation Rapid Automated Biodosimetry Tool II (RABiT-II) robotic system, a plate imager and custom software, FluorQuantDic. The system requires small volumes of blood (30 µl per individual) to determine radiation dose received as a result of a radiation accident or terrorist attack. To visualize dicentrics in multiwell plates, we implemented a non-classical protocol for centromere FISH staining at 37°C. The RABiT-II performs rapid analysis of chromosomes after extracting them from metaphase cells. With the use of multiwell plates, many samples can be screened at the same time. Thus, the RABiT-II DCA provides an advantage during triage when risk-based stratification and medical management are required for a large population exposed to unknown levels of ionizing radiation.
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