ArticleMethodsX2019
Easy calibration of the Light Plate Apparatus for optogenetic experiments.
Article in MethodsX, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
8 citing papers in PubMed.
- Targeted Activation of Programmed Cell Death Pathways by Optogenetics.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Transcription factor localization dynamics and DNA binding drive distinct promoter interpretations.Cell reports · 2023Article
- Article
- Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells.Journal of visualized experiments : JoVE · 2021Article
- Automated calibration of optoPlate LEDs to reduce light dose variation in optogenetic experiments.BioTechniques · 2020Article
- Proceedings of the 7th ACM International Conference on Nanoscale Computing and Communication : Virtual Conference, September 23-25, 2020 : NanoCom 2020. ACM International Conference on Nanoscale Computing and Communication (7th : 2020 :... · 2020Article
- A yeast optogenetic toolkit (yOTK) for gene expression control in Saccharomyces cerevisiae.Biotechnology and bioengineering · 2020Article
- Optogenetic Repressors of Gene Expression in Yeasts Using Light-Controlled Nuclear Localization.Cellular and molecular bioengineering · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Optogenetic systems use genetically-encoded light-sensitive proteins to control and study cellular processes. As the number and quality of these systems grows, there is an increasing need for user-friendly and flexible hardware to provide programmed illumination to cultures of cells. One platform which satisfies this need for a variety of optogenetic systems and organisms is the Light Plate Apparatus (LPA), which delivers a controlled light dose to each well of a 24-well plate. Experimental reproducibility requires appropriate calibration to produce accurate light doses within individual wells of the LPA and between LPAs. In this study, we present an easy and accurate method for calibrating the LPA. In particular, we: •developed a 3D printed adaptor and MATLAB code to allow rapid measurement of irradiance produced by the LPA and subsequent calibration•provide appropriate code and methodology for generating a standard curve for each LPA•demonstrate the utility and accuracy of this method between users and LPAs.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.