ArticleTranslational vision science & technology2019
Quantitative Discrimination of Healthy and Diseased Corneas With Second Harmonic Generation Microscopy.
Article in Translational vision science & technology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Two-photon imaging microscopy of non-stained human epiretinal membranes.Biomedical optics express · 2026Article
- Quantitative Characterization of Corneal Collagen Architecture Using Intensity Gradient Modeling and Gaussian PDF Fitting.Diagnostics (Basel, Switzerland) · 2025Article
- Structural changes in the crystalline lens as a function of the postmortem interval assessed with two-photon imaging microscopy.Biomedical optics express · 2024Article
- Analysis of age-related changes in the left ventricular myocardium with multiphoton microscopy.Biomedical optics express · 2024Article
- On the quantitative analysis of lamellar collagen arrangement with second-harmonic generation imaging.Biomedical optics express · 2024Article
- Decellularized squid mantle scaffolds as tissue-engineered corneal stroma for promoting corneal regeneration.Bioengineering & translational medicine · 2023Article
- Blind deconvolution of second harmonic microscopy images of the living human eye.Biomedical optics express · 2023Article
- Two-Photon Imaging for Non-Invasive Corneal Examination.Sensors (Basel, Switzerland) · 2022Review
- Assessment of the corneal collagen organization after chemical burn using second harmonic generation microscopy.Biomedical optics express · 2021Article
- Assessment of progressive alterations in collagen organization in the postoperative conjunctiva by multiphoton microscopy.Biomedical optics express · 2020Article
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3 authors.
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Abstract
purposeTo analyze the spatial organization of pathological corneas with second harmonic generation (SHG) imaging and to provide a proof of concept to objectively distinguish these from the healthy corneas.
methodsA custom-built SHG microscope was used to image the anterior stroma of ex vivo corneas, both control and affected by some representative pathologies. The structure tensor (ST) was employed as a metric to explore and quantify the alterations in the spatial distribution of the collagen lamellae.
resultsThe collagen arrangement differed between healthy and pathological samples. The former showed a regular distribution and a low structural dispersion (SD < 40°) within the stroma with a well-defined dominant orientation. This regular arrangement drastically turns into a disorganized pattern in pathological corneas (SD > 40°).
conclusionsThe combination of SHG imaging and the ST allows obtaining quantitative information to differentiate the stromal collagen organization in healthy and diseased corneas. This approach represents a feasible and powerful technique with potential applications in clinical corneal diagnoses. TRANSLATIONAL RELEVANCE: The ST applied to SHG microscopy images of the corneal stroma provides an experimental objective score to differentiate control from pathological or damaged corneas. Future implementations of this technique in clinical environments might might be a promising tool in Ophthalmology, not only to diagnose and monitor corneal diseases, but also to follow-up surgical outcome.
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