ArticleJournal of the American Chemical Society2019
Probing the Broad Time Scale and Heterogeneous Conformational Dynamics in the Catalytic Core of the Arf-GAP ASAP1 via Methyl Adiabatic Relaxation Dispersion.
Article in Journal of the American Chemical Society, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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9 citing papers in PubMed, 24 citations in OpenAlex.
- On the geometric phase effects on time evolution of the density matrix during modulated radiofrequency pulses.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2025Article
- Complete solution for rotating frame relaxation functions during adiabatic pulses.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2025Article
- Reduced dynamic complexity allows structure elucidation of an excited state of KRASCommunications biology · 2023Article
- Insights into the Cross Talk between Effector and Allosteric Lobes of KRAS from Methyl Conformational Dynamics.Journal of the American Chemical Society · 2022Article
- Theoretical classification of exchange geometries from the perspective of NMR relaxation dispersion.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2021Article
- Analysis of conformational exchange processes using methyl-TROSY-based Hahn echo measurements of quadruple-quantum relaxation.Magnetic resonance (Gottingen, Germany) · 2021Article
- The Role of Conformational Dynamics in the Recognition and Regulation of Ubiquitination.Molecules (Basel, Switzerland) · 2020Review
- Achieving pure spin effects by artifact suppression in methyl adiabatic relaxation experiments.Journal of biomolecular NMR · 2020Article
- Optimization of sortase A ligation for flexible engineering of complex protein systems.The Journal of biological chemistry · 2020Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Methyl-TROSY is one of the most powerful NMR spectroscopic tools for studying structures and conformational dynamics of large protein complexes in solution. In studying conformational dynamics, side chains usually display heterogeneous dynamics, including collective and local motions, that can be difficult to detect and analyze by conventional relaxation dispersion (RD) approaches. The combination of NH-based heteronuclear adiabatic relaxation dispersion (HARD) experiments and a geometric approximation (geoHARD) has been shown to have several advantages over conventional RD in revealing conformational dynamics over a broad time scale. Here, we demonstrate a new technique that has been developed to detect both heterogeneous and wide time scale conformational dynamics in the hydrophobic interior of large macromolecules utilizing methyl-geoHARD. It is shown that methyl-geoHARD will be feasible at ultrahigh magnetic fields (>1 GHz), when this technology becomes available. For the ZA domain of Arf-GAP ASAP1, with a global correlational time of 24 ns at 15 °C, a wide range of conformational dynamics (exhibiting chemical exchange rates (
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