Evidence map›Paper›PMID 31292818›Full record

ArticleJournal of assisted reproduction and genetics2019

A prospective study of non-invasive preimplantation genetic testing for aneuploidies (NiPGT-A) using next-generation sequencing (NGS) on spent culture media (SCM).

Queenie S Y Yeung, Ying Xin Zhang, Jacqueline P W Chung, Wai Ting Lui, Yvonne K Y Kwok, Baoheng Gui, Grace W S Kong, Ye Cao, Tin Chiu Li, Kwong Wai Choy

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of assisted reproduction and genetics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers, 4 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
50citing papers in PubMed, 4 pooled it
20.4field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

50 citing papers in PubMed, 4 syntheses or guidelines pooled it, 91 citations in OpenAlex.

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  7. Non-Invasive Preimplantation Genetic Testing (niPGT).Methods in molecular biology (Clifton, N.J.) · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Queenie S Y YeungAssisted Reproductive Technology Unit, Department of Obstetrics and Gynaecology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China.
Ying Xin ZhangDepartment of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Hong Kong SAR, China.
Jacqueline P W ChungAssisted Reproductive Technology Unit, Department of Obstetrics and Gynaecology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China.
Wai Ting LuiDepartment of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Hong Kong SAR, China.
Yvonne K Y KwokDepartment of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Hong Kong SAR, China.
Baoheng GuiShenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen, 518055, China.
Grace W S KongAssisted Reproductive Technology Unit, Department of Obstetrics and Gynaecology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China.
Ye CaoDepartment of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Hong Kong SAR, China.
Tin Chiu LiAssisted Reproductive Technology Unit, Department of Obstetrics and Gynaecology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China.
Kwong Wai ChoyDepartment of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Hong Kong SAR, China. richardchoy@cuhk.edu.hk.
Chinese University of Hong Kong · CNChinese University of Hong Kong, Shenzhen · CN

Funding

Research Grant Council of Hong Kong 14162417
6 · The paper itself

Abstract

purposeThis study was to evaluate if spent culture media (SCM) of embryos could be used as a non-invasive tool to achieve aneuploidy screening. Ploidy calls, as well as concordance rates between PGT-A results from trophectoderm (TE) and SCM, were compared. Clinical outcomes of single euploid transfers were also evaluated.

methodsThe study was conducted from March 2017 to June 2018 in a university-based ART center. SCM of day 3 to the day(s) of TE biopsy of all biopsied blastocysts were collected for testing. PGT-A results of SCM were compared with the standard results of TE, with clinical relevance and outcomes examined.

resultsNiPGT-A using SCM gave a sensitivity of 81.6%, specificity of 48.3%, positive predictive value of 82.6%, and negative predictive value of 46.7% in ploidy calling. The concordance rates for autosomes and sex determination were 62.1% and 82.4%, respectively. There were 14 single embryo transfer cycles of euploids as determined by TE biopsy. Clinical outcomes not only confirmed 3 false positive results from SCM but also reflected the true ploidy status of the transferred embryo in one case. If ploidy calls were dichotomized without mosaic embryos, the sensitivity and NPV would increase to 91.0% and 66.7% (p = 0.60 and p = 0.25), respectively.

conclusionsCell-free DNA found in SCM could provide ploidy information of an embryo as in PGT-A from its TE. Given its potential to reflect the comprehensive chromosomal profile of the whole embryo, more research based on clinical outcomes is required to determine if SCM could be a reliable selection tool in PGT-A.

Indexed as

AneuploidyFertilization in VitroCulture MediaEmbryo Culture TechniquesFemaleGenetic TestingHigh-Throughput Nucleotide SequencingHumansOocyte RetrievalOvulation InductionPregnancyPreimplantation DiagnosisProspective StudiesTrophoblastsCulture MediaAneuploidyNext-generation sequencingNiPGT-APGT-APreimplantation genetic testingSpent culture media

Identifiers

PMID31292818
PMCPMC6707994
OpenAlexW2956669571

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.