Evidence map›Paper›PMID 31292779›Full record

ArticleMikrochimica acta2019

An on-site bacterial detection strategy based on broad-spectrum antibacterial ε-polylysine functionalized magnetic nanoparticles combined with a portable fluorometer.

Xi Wu, Tiancheng Lai, Jiezhang Jiang, Yurou Ma, Guangyu Tao, Feng Liu, Na Li

Registry-linked trialAbstract read
PubMed Publisher
In one paragraph

Article in Mikrochimica acta, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT04864873 (An External Evaluation of a Metagenomic Next Generation Sequencing Workflow for Infection Diagnosis Using Oxford Nanopore Sequencing.), which is not on this map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.0field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT04864873 naunknown statusnot on this mapstarted 2021, after this paper: background citation

An External Evaluation of a Metagenomic Next Generation Sequencing Workflow for Infection Diagnosis Using Oxford Nanopore Sequencing.

TypeinterventionalSponsorCapital and Coast District Health boardRan2021 to 2022Enrolled400ConditionsBacterial InfectionsArmsMetagenomic next generation sequencing using Oxford Nanopore, Standard microbiological diagnostic pathway
3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 18 citations in OpenAlex.

  1. Article
  2. Article
  3. Electrochemical quantification of AgMikrochimica acta · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Xi Wu *Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China.
Tiancheng Lai *Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China.
Jiezhang JiangBeijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China.
Yurou MaBeijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China.
Guangyu TaoBeijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China.
Feng LiuBeijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China.
Na LiBeijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China. lina@pku.edu.cn.
Beijing National Laboratory for Molecular Sciences · CNPeking University · CN

Funding

National Natural Science Foundation of China 21475004National Natural Science Foundation of China 21535006
6 · The paper itself

Abstract

A sensitive on-site bacterial detection strategy is presented that integrates the broad-spectrum capturing feature of ε-polylysine-functionalized magnetic nanoparticles with an in-house built portable fluorometer. Based on the electrostatic interaction, the functionalized magnetic nanoparticles (ε-PL-MNPs) were prepared for Gram-positive and Gram-negative bacterial separation and subsequent viable release. ε-PL-MNPs show a broad reactivity towards bacteria with the high capture efficiency from real-world sample media. They also enable controlled viable bacterial release with pH adjustment. Detection of bacteria is based on a combination of broad-spectrum capture with colorimetric and fluorimetric immunoassays. A portable fluorometer is built to enhance the applicability for sensitive on-site detection. A limit of detection of 98 CFU·mL

Indexed as

BacteriaColorimetryFluorometryMagnetic PhenomenaNanoparticlesPolyethyleneiminePolylysinePolyethyleneiminePolylysineBroad-spectrum captureColorimetryControlled viable bacterial releaseFluorescenceImmunoassay

Identifiers

PMID31292779
OpenAlexW2959651667

What OpenQuestion holds

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Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.